Auxin-regulated reversible inhibition of TMK1 signaling by MAKR2 modulates the dynamics of root gravitropism
| Autor: | Lise C. Noack, Tom Beeckman, Jiří Friml, Laia Armengot, Zachary L. Nimchuk, Ana I. Caño-Delgado, Maria Mar Marquès-Bueno, Barbara K. Möller, Matthieu Pierre Platre, Davy Opdenacker, Vincent Bayle, Mengying Liu, Steffen Vanneste, Lesia Rodriguez, Yvon Jaillais, Joseph Bareille |
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| Přispěvatelé: | European Research Council, European Commission, Agence Nationale de la Recherche (France), Ministerio de Economía y Competitividad (España), National Science Foundation (US), Research Foundation - Flanders, Reproduction et développement des plantes (RDP), École normale supérieure - Lyon (ENS Lyon)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Institute of Science and Technology [Austria] (IST Austria), Vlaams Instituut voor Biotechnologie [Ghent, Belgique] (VIB), University of North Carolina [Chapel Hill] (UNC), University of North Carolina System (UNC), Centre for Research in Agricultural Genomics (CRAG), École normale supérieure de Lyon (ENS de Lyon)-Université Claude Bernard Lyon 1 (UCBL), Institute of Science and Technology [Klosterneuburg, Austria] (IST Austria) |
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
EFFLUX [SDV]Life Sciences [q-bio] PROTEIN Plant Roots chemistry.chemical_compound 0302 clinical medicine Loss of Function Mutation Arabidopsis CELL-SURFACE Brassinosteroid Auxin Receptor-like kinase chemistry.chemical_classification Anionic lipids biology food and beverages Plants Genetically Modified ARABIDOPSIS Transmembrane protein gravitropism Cell biology receptor-like kinase Gain of Function Mutation INTRACELLULAR TRAFFICKING Plant hormone Signal transduction ABP1 General Agricultural and Biological Sciences Gravitation Signal Transduction EXPRESSION ENDOCYTOSIS Gravitropism Protein Serine-Threonine Kinases anionic lipids Endocytosis General Biochemistry Genetics and Molecular Biology 03 medical and health sciences RHO ROP6 Report Indoleacetic Acids Arabidopsis Proteins fungi Membrane Proteins Biology and Life Sciences RECEPTOR-LIKE KINASES biology.organism_classification root TMK MAKR 030104 developmental biology chemistry Root brassinosteroid auxin 030217 neurology & neurosurgery |
| Zdroj: | CURRENT BIOLOGY Current Biology-CB Current Biology-CB, Elsevier, 2021, 31, pp.1-10. ⟨10.1016/j.cub.2020.10.011⟩ Dipòsit Digital de Documents de la UAB Universitat Autònoma de Barcelona Current Biology Current Biology-CB, 2021, 31, pp.1-10. ⟨10.1016/j.cub.2020.10.011⟩ Digital.CSIC. Repositorio Institucional del CSIC instname |
| ISSN: | 0960-9822 1879-0445 |
| Popis: | Summary Plants are able to orient their growth according to gravity, which ultimately controls both shoot and root architecture.1 Gravitropism is a dynamic process whereby gravistimulation induces the asymmetric distribution of the plant hormone auxin, leading to asymmetric growth, organ bending, and subsequent reset of auxin distribution back to the original pre-gravistimulation situation.1, 2, 3 Differential auxin accumulation during the gravitropic response depends on the activity of polarly localized PIN-FORMED (PIN) auxin-efflux carriers.1, 2, 3, 4 In particular, the timing of this dynamic response is regulated by PIN2,5,6 but the underlying molecular mechanisms are poorly understood. Here, we show that MEMBRANE ASSOCIATED KINASE REGULATOR2 (MAKR2) controls the pace of the root gravitropic response. We found that MAKR2 is required for the PIN2 asymmetry during gravitropism by acting as a negative regulator of the cell-surface signaling mediated by the receptor-like kinase TRANSMEMBRANE KINASE1 (TMK1).2,7, 8, 9, 10 Furthermore, we show that the MAKR2 inhibitory effect on TMK1 signaling is antagonized by auxin itself, which triggers rapid MAKR2 membrane dissociation in a TMK1-dependent manner. Our findings suggest that the timing of the root gravitropic response is orchestrated by the reversible inhibition of the TMK1 signaling pathway at the cell surface. Graphical Abstract Highlights • MAKR2 is co-expressed with PIN2 and regulates the pace of root gravitropism • MAKR2 controls PIN2 asymmetric accumulation at the root level during gravitropism • MAKR2 binds to and is a negative regulator of the TMK1 receptor kinase • Auxin antagonizes the MAKR2 inhibition of TMK1 by delocalizing MAKR2 in the cytosol Marquès-Bueno, Armengot et al. show that the unstructured protein MAKR2 controls the dynamics of the root gravitropic response by acting as a negative regulator of the TMK1 receptor kinase. In addition, the MAKR2 inhibitory effect on TMK1 signaling is antagonized by auxin itself, which triggers rapid MAKR2 membrane dissociation in a TMK1-dependent manner. |
| Databáze: | OpenAIRE |
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