Isolation and analysis of candidate myeloid tumor suppressor genes from a commonly deleted segment of 7q22
| Autor: | Brooke M. Emerling, Eric D. Green, Christian P. Kratz, Nicole P. Curtiss, Jason D. Balkman, Jennifer O. Lauchle, Michelle M. Le Beau, Jeannette M. Bonifas, Kevin Shannon |
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| Rok vydání: | 2005 |
| Předmět: |
Candidate gene
Monosomy DNA Complementary Tumor suppressor gene Pseudogene Molecular Sequence Data Biology Contig Mapping Gene mapping Genetics medicine Humans Genes Tumor Suppressor Cloning Molecular In Situ Hybridization Fluorescence Polymorphism Single-Stranded Conformational Chromosome 7 (human) Base Sequence Contig Reverse Transcriptase Polymerase Chain Reaction Computational Biology Sequence Analysis DNA Blotting Northern medicine.disease Neoplasm Proteins Trans-Activators Chromosome Deletion Bone Marrow Neoplasms Databases Nucleic Acid Chromosomes Human Pair 7 |
| Zdroj: | Genomics. 85:600-607 |
| ISSN: | 0888-7543 |
| DOI: | 10.1016/j.ygeno.2005.01.013 |
| Popis: | Monosomy 7 and deletions of 7q are recurring leukemia-associated cytogenetic abnormalities that correlate with adverse outcomes in children and adults. We describe a 2.52-Mb genomic DNA contig that spans a commonly deleted segment of chromosome band 7q22 identified in myeloid malignancies. This interval currently includes 14 genes, 19 predicted genes, and 5 predicted pseudogenes. We have extensively characterized the FBXL13, NAPE-PLD, and SVH genes as candidate myeloid tumor suppressors. FBXL13 encodes a novel F-box protein, SVHis a member of a gene family that contains Armadillo-like repeats, and NAPE-PLD encodes a phospholipase D-type phosphodiesterase. Analysis of a panel of leukemia specimens with monosomy 7 did not reveal mutations in these or in the candidate genes LRRC17, PRO1598, and SRPK2. This fully sequenced and annotated contig provides a resource for candidate myeloid tumor suppressor gene discovery. |
| Databáze: | OpenAIRE |
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