Isolation and characterization of lambda specialized transducing bacteriophages carrying Klebsiella pneumoniae nif genes
| Autor: | W J Brill, M M Howe, D MacNeil |
|---|---|
| Rok vydání: | 1980 |
| Předmět: |
Klebsiella pneumoniae
viruses Biology medicine.disease_cause Microbiology Bacteriophage mu Transduction (genetics) Plasmid Transduction Genetic Nitrogen Fixation Lysogenic cycle medicine Lysogeny Molecular Biology Escherichia coli Recombination Genetic Genetics biochemical phenomena metabolism and nutrition biology.organism_classification Bacteriophage lambda Genes Genetic Techniques DNA Transposable Elements bacteria Nif regulon Bacteriophage Mu Bacteria Research Article |
| Zdroj: | Journal of Bacteriology. 141:1264-1271 |
| ISSN: | 1098-5530 0021-9193 |
| DOI: | 10.1128/jb.141.3.1264-1271.1980 |
| Popis: | Seve lambda dnif specialized transducing bacteriophages were isolated from Escherichia coli strains containing plasmids carrying the his-nif region of Klebsiella pneumoniae. These phages collectively carry deoxyribonucleic acid for all of the genes in the nif regulon and adjacent deoxyribonucleic acid of K. pneumoniae. The phages were isolated by using Mu insertions in the nif region to direct the integration of lambda pMu phages in nif via formation of lambda pMu-Mu dilysogens which, upon induction, yielded lambda dnif phages. This procedure should be generally applicable for isolating lambda specialized transducing phages carrying genes from E. coli or other bacteria. |
| Databáze: | OpenAIRE |
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