Molecular and antigenic characterisation of ribosomal phosphoprotein P0 from Babesia bovis
| Autor: | Carlos Alberto do Nascimento Ramos, A. M. S. Sacco, Leucio C. Alves, G. M. S. Rosinha, Ingrid I. F. Souza, Flábio R. Araújo, Carina Elisei, Renato H.M. Oliveira, Cleber O. Soares |
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| Rok vydání: | 2009 |
| Předmět: |
Ribosomal Proteins
Microbiology (medical) Antigenicity lcsh:Arctic medicine. Tropical medicine lcsh:RC955-962 Protozoan Proteins lcsh:QR1-502 Antibodies Protozoan Cattle Diseases Antigens Protozoan recombinant ribosomal phosphoprotein lcsh:Microbiology law.invention Microbiology Apicomplexa Neospora law parasitic diseases Theileria medicine Animals Amino Acid Sequence Babesia bigemina biology bovine babesiosis Babesia bovis Babesiosis biology.organism_classification medicine.disease Virology Recombinant Proteins Immunoglobulin G Recombinant DNA Cattle Brazil |
| Zdroj: | Memórias do Instituto Oswaldo Cruz., Vol 104, Iss 7, Pp 998-1002 (2009) |
| ISSN: | 0074-0276 |
| Popis: | Babesia bovis is a tick-borne pathogen that remains an important constraint for the development of cattle industries in tropical and subtropical regions of the world. Effective control can be achieved by vaccination with live attenuated phenotypes of the parasite. However, these phenotypes have a number of drawbacks, which justifies the search for new, more efficient immunogens based mainly on recombinant protein technology. In the present paper, ribosomal phosphoprotein P0 from a Brazilian isolate of B. bovis was produced and evaluated with regard to conservation and antigenicity. The protein sequence displayed high conservation between different Brazilian isolates of B. bovis and several Apicomplexa parasites such as Theileria, Neospora and Toxoplasma. IgG from cattle experimentally and naturally infected with B. bovisas well as IgG1 and IgG2 from naturally infected cattle reacted with the recombinant protein. IgG from cattle experimentally infected with Babesia bigemina cross-reacted with B. bovis recombinant P0. These characteristics suggest that P0 is a potential antigen for recombinant vaccine preparations against bovine babesiosis. |
| Databáze: | OpenAIRE |
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