Osteogenic potential of sol-gel bioactive glasses containing manganese

Autor: Elizabeth Norris, Parichart Naruphontjirakul, Marivalda M. Pereira, Siwei Li, Julian R. Jones, Breno Rocha Barrioni
Rok vydání: 2019
Předmět:
Technology
Ceramics
Bone Regeneration
Cellular differentiation
02 engineering and technology
Bone tissue
BIOCOMPATIBILITY
law.invention
Engineering
0903 Biomedical Engineering
law
Osteogenesis
Materials Testing
Spectroscopy
Fourier Transform Infrared
Osteopontin
Materials Science
Biomaterials
biology
Cell Differentiation
021001 nanoscience & nanotechnology
Silicon Dioxide
STATE
medicine.anatomical_structure
Bioactive glass
Alkaline phosphatase
0210 nano-technology
BEHAVIOR
Materials science
SURFACE
Biocompatibility
Materials Science
0206 medical engineering
Biomedical Engineering
Biophysics
Bioengineering
Bone Marrow Cells
Phase Transition
MESENCHYMAL STEM-CELLS
Biomaterials
STRONTIUM
Calcification
Physiologic
XPS
medicine
Cell Adhesion
Humans
0912 Materials Engineering
Bone regeneration
Engineering
Biomedical
ANTIMICROBIAL ACTIVITY
Cell Proliferation
Ions
Manganese
Science & Technology
Osteoblasts
Mesenchymal stem cell
IN-VITRO BIOACTIVITY
Alkaline Phosphatase
020601 biomedical engineering
MN
Microscopy
Fluorescence
biology.protein
Glass
Zdroj: Journal of materials science. Materials in medicine. 30(7)
ISSN: 1573-4838
Popis: Bioactive glasses (BGs) are widely used for bone regeneration, and allow the incorporation of different ions with therapeutic properties into the glass network. Amongst the different ions with therapeutic benefits, manganese (Mn) has been shown to influence bone metabolism and activate human osteoblasts integrins, improving cell adhesion, proliferation and spreading. Mn has also been incorporated into bioceramics as a therapeutic ion for improved osteogenesis. Here, up to 4.4 mol% MnO was substituted for CaO in the 58S composition (60 mol% SiO2, 36 mol% CaO, 4 mol% P2O5) and its effects on the glass properties and capability to influence the osteogenic differentiation were evaluated. Mn-containing BGs with amorphous structure, high specific surface area and nanoporosity were obtained. The presence of Mn2+ species was confirmed by X-ray photoelectron spectroscopy (XPS). Mn-containing BGs presented no cytotoxic effect on human mesenchymal stem cells (hMSCs) and enabled sustained ion release in culture medium. hMSCs osteogenic differentiation stimulation and influence on the mineralisation process was also confirmed through the alkaline phosphatase (ALP) activity, and expression of osteogenic differentiation markers, such as collagen type I, osteopontin and osteocalcin, which presented higher expression in the presence of Mn-containing samples compared to control. Results show that the release of manganese ions from bioactive glass provoked human mesenchymal stem cell (hMSC) differentiation down a bone pathway, whereas hMSCs exposed to the Mn-free glass did not differentiate. Mn incorporation offers great promise for obtaining glasses with superior properties for bone tissue regeneration.
Databáze: OpenAIRE
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