Purification, characterization and comparison of phycoerythrins from three different marine cyanobacterial cultures
| Autor: | Datta Madamwar, Avani Kaushal, Asha Parmar, Sagar Sonawala, Niraj Kumar Singh |
|---|---|
| Rok vydání: | 2010 |
| Předmět: |
Cyanobacteria
Silver Staining Environmental Engineering Bioengineering Lyngbya Microbiology Bacterial Proteins Halomicronema β subunit Seawater Waste Management and Disposal biology Renewable Energy Sustainability and the Environment Phycoerythrin General Medicine biology.organism_classification Phormidium sp Fluorescence Spectrometry Fluorescence Biochemistry biology.protein Electrophoresis Polyacrylamide Gel Spectrophotometry Ultraviolet Stress conditions |
| Zdroj: | Bioresource technology. 102(2) |
| ISSN: | 1873-2976 |
| Popis: | The present study is focused on purification, characterization and comparison of phycoerythrins from three different marine cyanobacterial cultures--hormidium sp. A27 DM, Lyngbya sp. A09 DM and Halomicronema sp. A32 DM. 'Phycoerythrin' was successfully purified and characterized. On SDS-PAGE, the PE purified from all three young cultures showed four bands--corresponding to α and β subunits of each of PE-I and PE-II. However, phycoerythrin purified after prolonged growth of Phormidium sp. A27 DM and Halomicronema sp. A32DM showed only one band corresponding to 14 kDa whereas Lyngbya sp. A09 DM continued to produce uncleaved phycoerythrin. The absorption spectra of purified PEs from all the three young and old cultures showed variations however the fluorescence studies of the purified PEs in all cases gave the emission spectra at around 580 nm. The described work is of great importance to understand the role of phycoerythrin in adapting cyanobacteria to stress conditions. |
| Databáze: | OpenAIRE |
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