Glutathione pathway in ethylbenzene metabolism: Novel biomarkers of exposure in the rat
| Autor: | Frédéric Cosnier, Benoît Cossec, Stéphane Grossmann, Manuella Burgart, Hervé Nunge |
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| Rok vydání: | 2010 |
| Předmět: |
Male
Environmental Engineering Health Toxicology and Mutagenesis Urinary system Urine Ethylbenzene Rats Sprague-Dawley Excretion chemistry.chemical_compound Benzene Derivatives Animals Environmental Chemistry Inhalation Exposure Inhalation Public Health Environmental and Occupational Health Diastereomer General Medicine General Chemistry Metabolism Glutathione Pollution Rats chemistry Biochemistry Biomarkers Metabolic Networks and Pathways |
| Zdroj: | Chemosphere. 81:1334-1341 |
| ISSN: | 0045-6535 |
| Popis: | Glutathione pathway was specifically studied in rats exposed by inhalation to a range of ethylbenzene vapours (5–2000 ppm). Urines were collected during exposure (6 h) and over the 18 h following the exposure. The potential metabolites coming from either side-chain or ring oxidation were synthesized: 1-, 2-phenylethylmercapturic acids (1-, and 2-PEMA) and 2-, 3- and 4-ethylphenylmercapturic acids (2-, 3-, and 4-EPMA). Their synthesis was fully described and the molecules characterized. Urine samples were analysed using a selective HPLC–fluorescence method. Among the five metabolites, 2-PEMA was never observed in any urine sample. By contrast, 1-PEMA was discovered in its two diastereomeric forms, and it was shown that one of them was mainly present. 2-EPMA, 3-EPMA and 4-EPMA (in the ratio 1:2:6) were also found, and their combined excretion levels were similar to that of 1-PEMA. The atmospheric concentrations and urinary excretions yielded very close correlations which allow us to consider these mercapturic acids as novel ethylbenzene exposure biomarkers. |
| Databáze: | OpenAIRE |
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