Proteome analysis demonstrates profound alterations in human dendritic cell nature by TX527, an analogue of vitamin D
| Autor: | Evelyne van Etten, Lutgart Overbergh, Gabriela B Ferreira, Kasper Lage, Daniel Aaen Hansen, Etienne Waelkens, Yves Moreau, Christopher T. Workman, Chantal Mathieu, Mark Waer, Annemieke Verstuyf |
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| Rok vydání: | 2009 |
| Předmět: |
Proteome
CD14 Cell Blotting Western Cell Differentiation Dendritic cell Dendritic Cells Vitamins Biology Proteomics Biochemistry In vitro Cell biology medicine.anatomical_structure medicine Protein biosynthesis Humans Electrophoresis Gel Two-Dimensional Vitamin D Cytoskeleton Molecular Biology Cells Cultured |
| Zdroj: | Proteomics. 9(14) |
| ISSN: | 1615-9861 |
| Popis: | Structural analogues of vitamin D have been put forward as therapeutic agents able to exploit the immunomodulatory effects of vitamin D, without its undesired calcemic side effects. We have demonstrated that TX527 affects dendritic cell (DC) maturation in vitro, resulting in the generation of a tolerogenic cell. In the present study, we aimed to explore the global protein changes induced by the analogue in immature DC (iDC) and mature human DC and to correlate them with alterations in DC morphology and function. Human CD14 + monocytes were differentiated toward iDC or mature DCs, in the presence or absence of TX527 (10 -8 M) (n = 4). Protein samples were separated into two different pH ranges (pH4-7 and 6-9), analyzed by 2-D DIGE and differentially expressed spots (p |
| Databáze: | OpenAIRE |
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