Up-regulation of ectonucleotidase activity after cortical stab injury in rats
| Autor: | Aleksandar Vjestica, Mirjana Stojiljkovic, Sanja Pekovic, Danijela Stojkov, Ljubisav Rakic, Ivana Bjelobaba, Irena Lavrnja, Sanja Subasic, Nadezda Nedeljkovic |
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| Rok vydání: | 2005 |
| Předmět: |
Male
Adenosine Nucleotidase activity Cortical stab injury Hippocampus Rat brain 5'-nucleotidase 03 medical and health sciences 0302 clinical medicine Adenosine Triphosphate Antigens CD Nucleotidase medicine Ecto NTPDase Animals Ectonucleotidase 5'-Nucleotidase 030304 developmental biology 0303 health sciences Microglia Glial fibrillary acidic protein biology Apyrase fungi Ecto 50-nucleotidase Brain Cell Biology General Medicine Molecular biology Adenosine Monophosphate Rats Up-Regulation ATP Adenosine Diphosphate medicine.anatomical_structure Biochemistry Astrocytes Brain Injuries biology.protein 030217 neurology & neurosurgery |
| Zdroj: | Cell Biology International |
| ISSN: | 1065-6995 |
| Popis: | The objective of this study was to examine the changes in the activity and expression of ectonucleotidase enzymes in the model of unilateral cortical stab injury (CSI) in rat. The activities of ecto-nucleoside triphosphate diphosphohydrolase 1 (NTPDase 1) and ecto 5'-nucleotidase were assessed by measuring the levels of ATP, ADP and AMP hydrolysis in the crude membrane preparations obtained from injured left cortex, right cortex, left and right caudate nucleus, whole hippocampus and cerebellum. Significant increase in NTPDase and ecto 5'-nucleotidase activities was observed in the injured cortex following CSI, whereas in other brain areas only an increase in ecto 5'-nucleotidase activity was seen. Immunohistochemical analysis performed using antibodies specific to NTPDase 1 and ecto 5'-nucleotidase demonstrated that CSI induced significant changes in enzyme expression around the injury site. Immunoreactivity patterns obtained for NTPDase 1 and ecto 5'-nucleotidase were compared with those obtained for glial fibrillary acidic protein, as a marker of astrocytes and complement receptor type 3 (OX42), as a marker of microglia. Results suggest that up-regulation of ectonucleotidase after CSI is catalyzed by cells that activate in response to injury, i.e. cells immunopositive for NTPDase 1 were predominantly microglial cells, whereas cells immunopositive for ecto 5'-nucleotidase were predominantly astrocytes. |
| Databáze: | OpenAIRE |
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