Development of an improved time-resolved fluoroimmunoassay for simultaneous quantification of C-peptide and insulin in human serum
| Autor: | Jing-Yuan Hou, Tian-Cai Liu, Guanfeng Lin, Zhi-Qi Ren, Mei-Jun Chen, Ying-Song Wu |
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| Rok vydání: | 2014 |
| Předmět: |
medicine.medical_specialty
Time Factors medicine.medical_treatment Fluoroimmunoassay Clinical Biochemistry Sensitivity and Specificity Antibodies law.invention Sample volume chemistry.chemical_compound Limit of Detection law Internal medicine Diabetes mellitus Diabetes Mellitus Humans Insulin Medicine Chemiluminescence Chromatography C-Peptide medicine.diagnostic_test business.industry C-peptide Magnetic Phenomena Reproducibility of Results General Medicine Glucagon medicine.disease Highly sensitive Endocrinology Chronic disease chemistry Immunoassay Calibration business |
| Zdroj: | Clinical Biochemistry. 47:439-444 |
| ISSN: | 0009-9120 |
| DOI: | 10.1016/j.clinbiochem.2014.01.016 |
| Popis: | Objectives Diabetes mellitus is a chronic disease affecting millions of people globally and resulting in significant death rates each year. A fast, inexpensive alternative to traditional testing and monitoring techniques is desirable, since secretion of insulin and C-peptide is impaired in diabetes mellitus. Design and methods A highly sensitive immunoassay was developed for the simultaneous measurement of C-peptide and insulin levels in human serum, utilizing dual-label time-resolved fluoroimmunoassay (TRFIA) and magnetic particle technologies. This assay was characteristic for a single-step sandwich-type immunoassay, wherein antibody-coated magnetic particles were used as the solid phase and Eu 3 + and Sm 3 + chelate labels were used for detection. Results Antibody-coated magnetic particles in a TRFIA format performed well in addressing a number of quantitative needs. Conclusions The results of this assay correlated well with commercial chemiluminescence assays and provided a number a advantages, including reduced sample volume, reduced reagent and personnel costs and reduced assay time, while maintaining the required clinical sensitivity. |
| Databáze: | OpenAIRE |
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