In vivoinfection byTrypanosoma cruzi: The conserved FLY domain of the gp85/trans-sialidase family potentiates host infection
| Autor: | Ana Claudia Torrecilhas, Renata Rosito Tonelli, Maria A. Juliano, Walter Colli, J. F. Jacysyn, M. J. M. Alves |
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| Rok vydání: | 2010 |
| Předmět: |
CD4-Positive T-Lymphocytes
Chagas disease Trypanosoma cruzi Neuraminidase Parasitemia T-Lymphocytes Regulatory Mice In vivo medicine Animals Humans Chagas Disease Amino Acid Sequence IL-2 receptor Conserved Sequence Glycoproteins Mice Inbred BALB C Virulence biology Interleukin-2 Receptor alpha Subunit Kinetoplastida FOXP3 Forkhead Transcription Factors biology.organism_classification medicine.disease Virology Infectious Diseases biology.protein Female Animal Science and Zoology Parasitology Peritoneum Antibody Peptides Trypanosomiasis |
| Zdroj: | Parasitology. 138:481-492 |
| ISSN: | 1469-8161 0031-1820 |
| DOI: | 10.1017/s0031182010001411 |
| Popis: | SUMMARYTrypanosoma cruziis a protozoan parasite that infects vertebrates, causing in humans a pathological condition known as Chagas’ disease. The infection of host cells byT. cruziinvolves a vast collection of molecules, including a family of 85 kDa GPI-anchored glycoproteins belonging to the gp85/trans-sialidase superfamily, which contains a conserved cell-binding sequence (VTVXNVFLYNR) known as FLY, for short. Herein, it is shown that BALB/c mice administered with a single dose (1μg/animal, intraperitoneally) of FLY-synthetic peptide are more susceptible to infection byT. cruzi, with increased systemic parasitaemia (2-fold) and mortality. Higher tissue parasitism was observed in bladder (7·6-fold), heart (3-fold) and small intestine (3·6-fold). Moreover, an intense inflammatory response and increment of CD4+T cells (1·7-fold) were detected in the heart of FLY-primed and infected animals, with a 5-fold relative increase of CD4+CD25+FoxP3+T (Treg) cells. Mice treated with anti-CD25 antibodies prior to infection, showed a decrease in parasitaemia in the FLY model employed. In conclusion, the results suggest that FLY facilitatesin vivoinfection byT. cruziand concurs with other factors to improve parasite survival to such an extent that might influence the progression of pathology in Chagas’ disease. |
| Databáze: | OpenAIRE |
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