Mechanisms of abruption-induced premature rupture of the fetal membranes: Thrombin enhanced decidual matrix metalloproteinase-3 (stromelysin-1) expression
| Autor: | Frederick Schatz, Graciela Krikun, Charles J. Lockwood, Andrew P. Mackenzie, Edmund F. Funai, Susan Kadner |
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| Rok vydání: | 2004 |
| Předmět: |
Matrix Metalloproteinase 3
Fetal Membranes Premature Rupture medicine.medical_specialty Molecular Sequence Data Extraembryonic Membranes In Vitro Techniques Biology Matrix (biology) Sensitivity and Specificity Stromelysin 1 Thrombin Pregnancy Reference Values Fetal membrane Internal medicine Decidua medicine Humans Decidual cells RNA Messenger Cells Cultured Base Sequence Reverse Transcriptase Polymerase Chain Reaction Obstetrics and Gynecology Blotting Northern Blot Endocrinology Female Progestins Extracellular Matrix Degradation medicine.drug |
| Zdroj: | American Journal of Obstetrics and Gynecology. 191:1996-2001 |
| ISSN: | 0002-9378 |
| DOI: | 10.1016/j.ajog.2004.08.003 |
| Popis: | The aim of the study was to evaluate thrombin and progestin effects on matrix metalloproteinase-3 expression in term decidual cells as a mechanism of abruption-related preterm delivery.Decidual cells were isolated by standard techniques, purified to homogeneity, grown to confluence, and passaged. Cultures were primed with 10 (-8) M estradiol or estradiol plus 10 (-7) progestin and then incubated in a defined medium with corresponding steroid(s) plus or minus thrombin or the protease-activated thrombin receptor-1 agonist for 24 hours. Secreted matrix metalloproteinase-3 levels were assessed by enzyme-linked immunosorbent assay, and immunoblotting and messenger RNA levels were measured by Northern blotting and quantitative reverse transcription-polymerase chain reaction.Immunoreactive matrix metalloproteinase-3 levels were inhibited 66% by estradiol plus progestin versus estradiol ( P.05). Thrombin elicited a dose-dependent reversal in this progestin inhibition, producing a 2.5-fold increase at 2.5 U/mL ( P.05) that attained 33% of matrix metalloproteinase-3 levels in parallel incubations with estradiol plus thrombin. Protease-activated thrombin receptor-1 agonist mimicked 60% of thrombin-enhanced matrix metalloproteinase-3 output. Immunoblotting validated the enzyme-linked immunosorbent assay results. Northern blotting and quantitative reverse transcription-polymerase chain reaction demonstrated corresponding effects on steady-state messenger RNA levels.Abruption-generated thrombin promotes preterm delivery by mediating fetal membrane extracellular matrix degradation via enhanced decidual cell matrix metalloproteinase-3 expression, whereas progesterone blunts this thrombin-induced effect. |
| Databáze: | OpenAIRE |
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