Use of FTA® card methodology for sampling and molecular characterization of Echinococcus granulosus sensu lato in Africa

Autor: Ahmed Bezeid El Mamy, Ikhlass El Berbri, Gérald Umhang, Jean-Marc Boucher, Allal Dakkak, Ouafaa Fassi Fihri, Franck Boué, Abdallah Traore, Anne-Françoise Petavy, Vanessa Hormaz
Přispěvatelé: Laboratoire de la rage et de la faune sauvage de Nancy (LRFSN), Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES), Department of Pathology and Veterinary Public Health, National Center for Livestock and for Veterinary Research, Central Veterinary Laboratory, Université Claude Bernard Lyon 1 - Faculté des sciences et technologies (UCBL FST), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon, European Project: 221948,EC:FP7:KBBE,FP7-KBBE-2007-2A,ICONZ(2009), Université Claude Bernard Lyon 1 - Faculté des sciences (UCBL FS)
Jazyk: angličtina
Rok vydání: 2017
Předmět:
Zdroj: Experimental Parasitology
Experimental Parasitology, Elsevier, 2017, 173, pp.29-33. ⟨10.1016/j.exppara.2016.12.016⟩
Experimental Parasitology, 2017, 173, pp.29-33. ⟨10.1016/j.exppara.2016.12.016⟩
ISSN: 0014-4894
1090-2449
DOI: 10.1016/j.exppara.2016.12.016⟩
Popis: International audience; Cystic Echinococcosis is a parasitic disease caused by the cestode Echinococcus granulosus widely distributed in Africa. Monitoring of this parasite requires access to cyst samples on intermediate hosts observed at the slaughterhouse. In order to facilitate sampling in the field and analysis, the French National Reference Laboratory for Echinococcus spp. has developed a tissue derived from DNA sampling with FTA® card technology. The DNA samples were taken by applying the FTA® paper on the germinal layer after opening the cysts. The sampling technique was validated using frozen cysts (n = 76) stored in the laboratory and from field samples (n = 134) taken at the slaughterhouse by veterinarian technicians during meat inspection in Morocco, Mali and Mauritania. DNA was extracted after several weeks of storage at room temperature. PCR assays were performed using primers for generic cestode (cox1) and amplified fragments were sequenced. All samples taken in the lab and 80% of field samples were capable of molecular characterization. Cyst-derived DNA from FTA® samples can be useful for easy sampling, storage and rapid, safe and cheap shipment. The use of the FTA methodology will facilitate studies in the field to investigate the presence and genetic characterization of E. granulosus sensu lato in African countries.
Databáze: OpenAIRE
Externí odkaz: