Diverse spinal commissural neuron populations revealed by fate mapping and molecular profiling using a novel Robo3
| Autor: | Alexander Jaworski, Brigett V Carvajal, Marc Tessier-Lavigne, Alastair J. Tulloch, Shaun Teo |
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| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Nervous system Male Cre recombinase Mice Transgenic Receptors Cell Surface Biology Commissural Interneurons Article 03 medical and health sciences Mice 0302 clinical medicine Fate mapping medicine Animals Neurons Integrases General Neuroscience Gene Expression Profiling Chromosome Mapping Commissure Spinal cord Phenotype 030104 developmental biology medicine.anatomical_structure nervous system Spinal Cord Axon guidance Female Neuron Neuroscience 030217 neurology & neurosurgery |
| Zdroj: | J Comp Neurol |
| ISSN: | 1096-9861 |
| Popis: | The two sides of the nervous system coordinate and integrate information via commissural neurons, which project axons across the midline. Commissural neurons in the spinal cord are a highly heterogeneous population of cells with respect to their birthplace, final cell body position, axonal trajectory, and neurotransmitter phenotype. Although commissural axon guidance during development has been studied in great detail, neither the developmental origins nor the mature phenotypes of commissural neurons have been characterized comprehensively, largely due to lack of selective genetic access to these neurons. Here, we generated mice expressing Cre recombinase from the Robo3 locus specifically in commissural neurons. We used Robo3(Cre) mice to characterize the transcriptome and various origins of developing commissural neurons, revealing new details about their extensive heterogeneity in molecular makeup and developmental lineage. Further, we followed the fate of commissural neurons into adulthood, thereby elucidating their settling positions and molecular diversity and providing evidence for possible functions in various spinal cord circuits. Our studies establish an important genetic entry point for further analyses of commissural neuron development, connectivity, and function. |
| Databáze: | OpenAIRE |
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