Moraxella species: infectious microbes identified by use of time-of-flight mass spectrometry
| Autor: | Yuko Asano, Hiroki Yamada, Hideaki Kawakami, Kiyofumi Mochizuki, Kazuhiro Murata, Kenji Ozawa, Shunsuke Takahashi, Asami Nakayama, Hiroshige Mikamo |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
Adult
Male medicine.medical_specialty Adolescent medicine.drug_class Moraxellaceae Infections Antibiotics Cefazolin Ceftazidime Eye Infections Bacterial Mass Spectrometry Keratitis Cornea Young Adult 03 medical and health sciences 0302 clinical medicine Levofloxacin medicine Tobramycin Humans Moraxella Child Aged Retrospective Studies Aged 80 and over biology business.industry Infant Reproducibility of Results General Medicine Middle Aged Conjunctivitis medicine.disease biology.organism_classification Dermatology Ophthalmology Child Preschool 030221 ophthalmology & optometry Ceftriaxone Female business Conjunctiva 030217 neurology & neurosurgery medicine.drug |
| Zdroj: | Japanese Journal of Ophthalmology. 63:328-336 |
| ISSN: | 1613-2246 0021-5155 |
| Popis: | To report the clinical manifestations, identification, antimicrobial susceptibilities, and treatment outcomes of ocular infections caused by Moraxella species. Retrospective study. The medical records of all patients treated at the Departments of Ophthalmology of the Ogaki Municipal Hospital and the Gifu University Graduate School of Medicine for ocular infections caused by Moraxella species between January 2011 and June 2017 were examined. The stored Moraxella species isolated from ocular samples were identified by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS), molecular identification, and the biochemical properties. Sixteen eyes of 16 patients were treated for Moraxella ocular infections. The patients’ median age was 72 years. A predisposing systemic or ocular condition was identified in 15 of the patients. Nine of the patients developed keratitis; four, conjunctivitis; and three, blebitis. M lacunata (6 eyes), M catarrhalis (6), M nonliquefaciens (3), and M osloensis (1) were identified by MALDI-TOF MS. All isolates were sensitive to levofloxacin, tobramycin, ceftazidime, ceftriaxone, and cefazolin. Twelve patients with keratitis or blebitis were treated with various topical antimicrobial combinations, and systemic antibiotics were used in 10 of the 12 patients. The mean time for the complete closure of the epithelial defects with keratitis was 24 days. The visual outcomes after treatment were favorable except in 1 keratitis patient who underwent enucleation. The use of duo-therapy with a combination of fluoroquinolone and cefmenoxime should be considered in cases nonresponsive to monotherapy, such as keratitis and bleb-associated infections. MALDI-TOF MS is useful for the identification of Moraxella to the species level. |
| Databáze: | OpenAIRE |
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