Exosomes Derived from Platelet-Rich Plasma Administration in Site Mediate Cartilage Protection in Subtalar Osteoarthritis
| Autor: | Tao Jiang, Tao Xu, Ziyang Zheng, Tao Qin, Zheng Zhou, Yin Guoyong, Qingqing Li, Dingfei Qian, Tianyi Bao, Yifan Huang, Yu Zhang, Jian Chen, Shujie Zhao, Jin Fan, Xuan Zhao, Peng Gao, Qian Wang, Jiang Yi, Hao Liu, Xiaowei Wang |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
Biomedical Engineering
Pharmaceutical Science Medicine (miscellaneous) Bioengineering macromolecular substances Osteoarthritis Pharmacology Exosomes Applied Microbiology and Biotechnology Mice Platelet-rich plasma Medical technology medicine Animals Humans In situ drug delivery R855-855.5 business.industry Research Cartilage fungi Subtalar osteoarthritis medicine.disease Microvesicles Exosome carbohydrates (lipids) medicine.anatomical_structure Quality of Life Molecular Medicine business TP248.13-248.65 Biotechnology |
| Zdroj: | Journal of Nanobiotechnology Journal of Nanobiotechnology, Vol 20, Iss 1, Pp 1-22 (2022) |
| Popis: | Subtalar osteoarthritis (STOA) is often secondary to chronic ankle sprains, which seriously affects the quality of life of patients. Due to its etiology and pathogenesis was not studied equivocally yet, there is currently a lack of effective conservative treatments. Although they have been used for tissue repair, platelet-rich plasma-derived exosomes (PRP-Exo) have the disadvantage of low retention and short-lived therapeutic effects. This study aimed to determine whether incorporation of PRP-Exo in thermosensitive hydrogel (Gel) increased their retention in the joint and thereby playing a therapeutic role on STOA due to chronic mechanical instability established by transecting lateral ligaments (anterior talofibular ligament (ATFL)/calcaneal fibular ligament (CFL)). PRP-Exo incorporated Gel (Exo-Gel) system, composed of Poloxamer-407 and 188 mixture-based thermoresponsive hydrogel matrix in an optimal ratio, was determined by its release ability of Exo and rheology of Gel response to different temperature. The biological activity of Exo-Gel was evaluated in vitro, and the therapeutic effect of Exo-Gel on STOA was evaluated in vivo. Exo released from Exo-Gel continuously for 28 days could promote the proliferation and migration of mouse bone mesenchymal stem cells (mBMSCs) and chondrocytes, at the same time enhance the chondrogenic differentiation of mBMSCs, and inhibit inflammation-induced chondrocyte degeneration. In vivo experiments confirmed that Exo-Gel increased the local retention of Exo, inhibited the apoptosis and hypertrophy of chondrocytes, enhanced their proliferation, and potentially played the role in stem cell recruitment to delay the development of STOA. Thus, Delivery of PRP-Exo incorporated in thermosensitive Gel provides a novel approach of cell-free therapy and has therapeutic effect on STOA. Graphical Abstract Supplementary Information The online version contains supplementary material available at 10.1186/s12951-022-01245-8. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|