A new blocking ELISA for detection of foot-and-mouth disease non-structural protein (NSP) antibodies in a broad host range

Autor: Madhusudan Hosamani, Shreya Gopinath, B. P. Sreenivasa, Subhasmita Behera, Suresh H. Basagoudanavar, Ashok Boora, Durlav P. Bora, Pankaj Deka, Veerakyathappa Bhanuprakash, Raj Kumar Singh, Aniket Sanyal, Klaas Weerdmeester, Aldo Dekker
Rok vydání: 2021
Předmět:
Zdroj: Applied Microbiology and Biotechnology 106 (2022)
Applied Microbiology and Biotechnology, 106, 6745-6757
ISSN: 1432-0614
0175-7598
Popis: Large-scale monitoring of foot-and-mouth disease (FMD) in livestock is imperative in an FMD control program. Detection of antibodies against non-structural proteins (NSP) of FMD virus (FMDV) is one of the best tools to estimate the prevalence of past infection; availability of such a well-validated test is therefore essential. Using a FMDV 3B protein-specific monoclonal antibody, we have developed a new NSP antibody blocking ELISA (10H9 bELISA) and validated it on large panels of sera from different susceptible species. The diagnostic sensitivity of the ELISA was 95% with a specificity of 98%, similar to the values found using a commercial kit (PrioCHECK FMD NS test). The 10H9 bELISA can be used in a broad range of FMD susceptible species making it a very useful tool in monitoring the foot-and-mouth disease control programs by detection of virus circulation in the vaccinated populations. Key points: • A new ELISA for detection of foot and mouth disease (FMD) antibodies. • Diagnostic sensitivity of 95% and specificity of 98%. • Tested with panels of validated sera from broad host range.
Databáze: OpenAIRE
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