Cytotoxic T lymphocytes promote cytarabine-induced acute myeloid leukemia cell apoptosis via inhibiting Bcl-2 expression
| Autor: | Yan Zeng, Yi Su, Yan‑Rong Shuai, Yi Wang, Hai Yi, Li Fu, Rui Deng, Fang‑Yi Fan, Xiao‑Juan Miao, Guang‑Cui He |
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| Rok vydání: | 2017 |
| Předmět: |
Cancer Research
acute myeloid leukemia cells CD3 medicine.medical_treatment chemical and pharmacologic phenomena cytotoxic T lymphocytes 03 medical and health sciences 0302 clinical medicine Immunology and Microbiology (miscellaneous) cytarabine hemic and lymphatic diseases medicine Cytotoxic T cell B-cell lymphoma 2 biology Myeloid leukemia Articles General Medicine Immunotherapy medicine.disease CTL Leukemia 030220 oncology & carcinogenesis Immunology Cytarabine biology.protein Cancer research immunotherapy CD8 030215 immunology medicine.drug |
| Zdroj: | Experimental and Therapeutic Medicine |
| ISSN: | 1792-1015 1792-0981 |
| DOI: | 10.3892/etm.2017.4620 |
| Popis: | Acute myeloid leukemia (AML) remains difficult to cure due to its drug tolerance and refractoriness. Immunotherapy is a growing area of cancer research, which has been applied for the treatment of numerous types of cancer, including leukemia. The present study generated AML cell-specific cytotoxic T lymphocytes (CTLs) in vitro and investigated the effect of combining CTL treatment with one of the most commonly used drugs for the treatment of hematological malignancies, cytarabine, on AML cell apoptosis. Firstly, it was observed that monocyte-depleted peripheral blood lymphocytes from healthy donors could be used to generate large numbers of CD3+CD8+ CTLs through immune stimulation. These CD3+CD8+ CTLs could effectively recognize and induce the apoptosis of human Kasumi-3 AML cells. In addition, cytarabine-induced AML cell apoptosis was enhanced by CTL treatment. Western blotting revealed that Bcl-2 expression was downregulated in AML cells following cytarabine and CTL treatment, indicating that the synergistic effect of this treatment on AML cell apoptosis is due to the downregulation of Bcl-2. These results highlight the potential application of CTL immunotherapy for the treatment of AML. Further studies optimizing the specificity and potency of CTLs, and identifying favorable combinations with other chemotherapeutic drug are required. |
| Databáze: | OpenAIRE |
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