Preadipocytes stimulate breast cancer cell growth
| Autor: | David Heber, K Setoodeh, Dilprit Bagga, Elena Elstner, H P Koeffler, Hilda Chamras |
|---|---|
| Rok vydání: | 1998 |
| Předmět: |
Cancer Research
medicine.medical_specialty Cellular differentiation Medicine (miscellaneous) Adipose tissue Breast Neoplasms Biology medicine.disease_cause Mice Feeder Layer Cell–cell interaction Internal medicine Adipocytes Tumor Cells Cultured medicine Animals Humans Nutrition and Dietetics Cell growth Stem Cells Cell Differentiation 3T3 Cells Coculture Techniques Endocrinology Oncology Cell culture Cancer cell Cancer research Carcinogenesis Cell Division |
| Zdroj: | Nutrition and Cancer. 32:59-63 |
| ISSN: | 1532-7914 0163-5581 |
| DOI: | 10.1080/01635589809514719 |
| Popis: | Mammary fat may affect human breast cancer development. In this study a novel coculture system was used to investigate stromal-epithelial cell interactions in vitro. The extent of differentiation of a feeder layer of 3T3-L1 preadipocytes affected the clonal growth of breast cancer cells under anchorage-independent conditions. When the feeder-layer preadipocytes were proliferating, the clonal growth of estrogen receptor-positive MCF-7 and estrogen receptor-negative MDA-MB-231 and MDA-MB-436 cells was stimulated by 100%, 43%, and 60%, respectively. In contrast, after differentiation the feeder layer of mature adipocytes inhibited clonal growth of breast cancer cell lines by 60%. No clonal growth stimulation of human pancreatic (Panc-1) or prostatic (PC-3) cancer cells was noted when these cells were grown under identical conditions with a proliferating 3T3-L1 cell feeder layer. These findings suggest that proliferating preadipocytes may be associated with factors that specifically stimulate breast cancer cell growth. |
| Databáze: | OpenAIRE |
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