EVOLUTION AND MITOCHONDRIAL DNA IN NEUROSPORA CRASSA
| Autor: | Georgiana May, John W. Taylor, Beverly D. Smolich |
|---|---|
| Rok vydání: | 1986 |
| Předmět: |
0106 biological sciences
0301 basic medicine Genetics Mitochondrial DNA biology Crassa biology.organism_classification 010603 evolutionary biology 01 natural sciences Genome Neurospora crassa 03 medical and health sciences Restriction enzyme 030104 developmental biology Molecular evolution General Agricultural and Biological Sciences Gene Genome size Ecology Evolution Behavior and Systematics |
| Zdroj: | Evolution. 40:716-739 |
| ISSN: | 1558-5646 0014-3820 |
| DOI: | 10.1111/j.1558-5646.1986.tb00533.x |
| Popis: | We studied mitochondrial DNA variability in 19 natural Neurospora crassa isolates and one wild-type isolate to examine evolution of these fungi and their mitochondrial DNA (mtDNA). We combined restriction endonuclease analysis of natural isolate mtDNA with DNA-DNA hybridization to cloned EcoR I fragments of a wild-type genome to discriminate between length mutations and site changes due to nucleotide substitution. Most variability was due to length mutations (insertions and deletions); genome size could vary 25% between pairs of isolates. Length-mutation distribution was not random, nor simply explained by the presence of coding versus noncoding regions. Restriction-site changes were few; the estimated amount of nucleotide substitution per nucleotide between the most divergent pair of isolates was 0.78%. Evolutionary relationships among isolates based on both types of mutations were compatible, and suggest that geographically distinct populations of mitochondrial DNA exist in the biological species, N. crassa. In contrast, no such correlation was shown by the previously determined distribution of nuclear heterokaryon incompatibility genes in the same isolates (Mylyk, 1975, 1976). |
| Databáze: | OpenAIRE |
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