A Biological Assay for Ribonuclease with Sub-millimicrogram Sensitivity

Autor:	H. L. Bachrach, J. Polatnick
Rok vydání:	1960
Předmět:	chemistry.chemical_classification Virus quantification Infectivity Serial dilution biology RNase P RNA Molecular biology General Biochemistry Genetics and Molecular Biology Virus Ribonucleases Enzyme chemistry Biochemistry biology.protein Biological Assay Ribonuclease
Zdroj:	Experimental Biology and Medicine. 105:486-489
ISSN:	1535-3699 1535-3702
DOI:	10.3181/00379727-105-26151
Popis:	SummaryAn ultra-sensitive method of assay for ribonuclease (RNase) has been devised using a biologically active ribonucleic acid (RNA) as its substrate. Infectious RNA isolated from foot-and-mouth disease virus was exposed to suitable dilutions of RNase, and the resulting loss in RNA infectivity was measured by plaque assay in calf-kidney cultures. Concentrations of enzyme as low as 0.1 mμg per ml were readily detected. The RNase level in sera from man and several animal species was determined by this method. Most sera contained 0.02 to 0.1 μg/ml of RNase, but guinea pig serum had a 20- to 30-fold higher content of this enzyme.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::05c1edaf479e1ef56499779e96e93c6d https://doi.org/10.3181/00379727-105-26151 Zobrazit plný text záznamu