Productive infection of primary murine astrocytes, lymphocytes, and macrophages by human immunodeficiency virus type 1 in culture
| Autor: | Jadwiga Nitkiewicz, Gabrielle Grunig, So Young Choi, Jinliang Li, Mary Jane Potash, Seon-Young Kim, Wei Chao, David J. Volsky, Galina Bentsman, Harris A. Gelbard |
|---|---|
| Rok vydání: | 2005 |
| Předmět: |
viruses
RNA Splicing Blotting Western HIV Core Protein p24 HIV Infections Virus Replication Virus Cellular and Molecular Neuroscience Mice Viral Envelope Proteins Viral entry Virology Murine leukemia virus medicine Animals Lymphocytes Membrane Glycoproteins biology Reverse Transcriptase Polymerase Chain Reaction Macrophages biology.organism_classification Disease Models Animal medicine.anatomical_structure Neurology Viral replication Cell culture Vesicular stomatitis virus Astrocytes HIV-1 NIH 3T3 Cells Neuroglia RNA Viral Female Neurology (clinical) Viral disease |
| Zdroj: | Journal of neurovirology. 10(6) |
| ISSN: | 1355-0284 |
| Popis: | A mouse model of human immunodeficiency virus type 1 (HIV-1) infection would be extremely valuable for evaluation of therapies and vaccines; however, multiple blocks to productive infection of NIH 3T3 and other mouse cell lines have been reported. The authors investigated the replication of HIV-1 in primary mouse astrocytes, lymphocytes, and macrophages in culture by infection with intact HIV-1 pseudotyped with the vesicular stomatitis virus G envelope glycoprotein (VSV-G) or with the envelope glycoprotein of amphotropic murine leukemia virus. Astrocytes, lymphocytes, and macrophages were susceptible to productive infection as variously assayed by detection of p24 and Tat proteins, viral protease-mediated processing of Gag, appropriately spliced viral RNA, and infectious progeny virus. As expected, NIH 3T3 cells were not susceptible to productive infection by VSV/NL4. Susceptibility mapped neither to the Fv locus nor to a possible polymorphism in cyclin T1. This study indicates that there are no intrinsic intracellular barriers to HIV-1 replication in primary mouse cells when virus entry is efficient. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full text from SpringerLink