Effects of downregulating TEAD4 transcripts by RNA interference on early development of bovine embryos
| Autor: | Kazuki Takahashi, Nobuyuki Sakurai, Ken Sawai, Tsutomu Hashizume, Natsuko Emura |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Homeobox protein NANOG Down-Regulation Embryonic Development Gene Expression Muscle Proteins GATA3 Transcription Factor Biology Pregnancy Proteins 03 medical and health sciences 0302 clinical medicine RNA interference Gene expression medicine Animals CDX2 Transcription Factor TEAD4 Blastocyst CDX2 Transcription factor Messenger RNA 030219 obstetrics & reproductive medicine POU domain Bovine embryo Molecular biology Cell biology DNA-Binding Proteins Preimplantation development 030104 developmental biology medicine.anatomical_structure embryonic structures Interferon Type I Animal Science and Zoology Cattle Female Original Article Octamer Transcription Factor-3 Transcription Factors |
| Zdroj: | The Journal of Reproduction and Development |
| ISSN: | 1348-4400 |
| Popis: | Transcription factor TEA domain family transcription factor 4 (Tead4) is one of the key factors involved in the differentiation of the trophectoderm (TE) in murine embryos. However, knowledge on the roles of TEAD4 in preimplantation development during bovine embryos is currently limited. This study examined the transcript and protein expression patterns of TEAD4 and attempted to elucidate the functions of TEAD4 during bovine preimplantation development using RNA interference. TEAD4 mRNA was found to be upregulated between the 16-cell and morula stages, and nuclear localization of the TEAD4 protein was detected at the morula stage, as well as in subsequent developmental stages. TEAD4 downregulation did not affect embryonic development until the blastocyst stage, and TEAD4-downregulated embryos were capable of forming the TE under both 5% and 21% O2 conditions. Results of gene expression analysis showed that TEAD4 downregulation did not affect the expression levels of POU class 5 transcription factor 1 (OCT-4), NANOG, caudal-type homeobox 2 (CDX2), GATA binding protein 3 (GATA3), and interferon-tau (IFNT). In conclusion, TEAD4 might be dispensable for development until the blastocyst stage and TE differentiation in bovine embryos. |
| Databáze: | OpenAIRE |
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