Cardiac Myocyte Excitation by Ultrashort High-Field Pulses
| Autor: | P. Thomas Vernier, Jiexiao Chen, Martin A. Gundersen, Miguel Valderrábano, Meng-Tse Chen, Sufen Wang |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2009 |
| Předmět: |
medicine.medical_specialty
Thapsigargin Biophysics 02 engineering and technology Tetrodotoxin Membrane Potentials Rats Sprague-Dawley 03 medical and health sciences chemistry.chemical_compound Internal medicine Caffeine medicine Myocyte Animals Myocytes Cardiac Calcium Signaling Ion channel Cells Cultured 030304 developmental biology Membrane potential 0303 health sciences Chemistry Ryanodine receptor Ryanodine Thiourea 021001 nanoscience & nanotechnology Calcium Channel Blockers Electric Stimulation Rats Electrophysiology Endocrinology Verapamil Calcium 0210 nano-technology Extracellular Space Intracellular medicine.drug Sodium Channel Blockers |
| Popis: | In unexcitable, noncardiac cells, ultrashort (nanosecond) high-voltage (megavolt-per-meter) pulsed electrical fields (nsPEF) can mobilize intracellular Ca2+ and create transient nanopores in the plasmalemma. We studied Ca2+ responses to nsPEF in cardiac cells. Fluorescent Ca2+ or voltage signals were recorded from isolated adult rat ventricular myocytes deposited in an electrode microchamber and stimulated with conventional pulses (CPs; 0.5–2.4 kV/cm, 1 ms) or nsPEF (10–80 kV/cm, 4 ns). nsPEF induced Ca2+ transients in 68/104 cells. Repeating nsPEF increased the likelihood of Ca2+ transient induction (61.8% for |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|