Examination of the Reticular Epithelium of the Bovine Pharyngeal Tonsil
| Autor: | Tyler C. Thacker, Mitchell V. Palmer, W. Ray Waters, Judith A. Stasko |
|---|---|
| Rok vydání: | 2011 |
| Předmět: |
CD4-Positive T-Lymphocytes
Male Nasal cavity Pathology medicine.medical_specialty Histology Pharyngeal Tonsil Biology Adenoid Epithelium Organ Culture Techniques Microscopy Electron Transmission Phagocytosis Antigen medicine Animals Ecology Evolution Behavior and Systematics B-Lymphocytes Epithelial Cells Receptors Antigen T-Cell gamma-delta Immunohistochemistry CD11c Antigen medicine.anatomical_structure Tonsil Adenoids Reticular connective tissue Microscopy Electron Scanning Respiratory epithelium Cattle Anatomy Biotechnology |
| Zdroj: | The Anatomical Record: Advances in Integrative Anatomy and Evolutionary Biology. 294:1939-1950 |
| ISSN: | 1932-8486 |
| DOI: | 10.1002/ar.21448 |
| Popis: | The pharyngeal tonsil (adenoid), located at the posterior of the nasopharynx is ideally positioned to sample antigens passing through the nasal cavity or oral cavity. Entering antigens will first contact tonsilar epithelium. To better understand the cellular organization of this important epithelial layer, pharyngeal tonsils were collected from six, 7-month-old calves and examined by light microscopy, immunohistochemistry, and electron microscopy. Morphometric analysis showed that the epithelium overlying lymphoid follicles (reticular epithelium) contained significantly more B-cells, CD4+, and CD11c+ cells than nonreticular epithelium. In contrast, nonreticular epithelium contained significantly more, γ/δ TCR+ cells than reticular epithelium. Scanning and transmission electron microscopy of reticular epithelium identified a heterogeneous population of epithelial cells, many of which displayed morphologic characteristics of M-cells. Moreover, putative M-cells were shown to possess the capacity for microparticle uptake. Bovine pharyngeal tonsilar reticular epithelium contains key immune cells, as well as M-cells; elements essential for antigen uptake, antigen processing, and initiation of immune responses. A better understanding of the morphology and function of tonsilar lymphoepithelium will strengthen our understanding of it's role in disease pathogenesis, and potential use as an induction site for mucosal immune responses to vaccination. Anat Rec, 2011. © 2011 Wiley-Liss, Inc. |
| Databáze: | OpenAIRE |
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