Penetration of C14-labelled mannitol from serum into cerebrospinal fluid and brain
| Autor: | Burton L. Wise, Elizabeth Stevenson, Kenneth G. Scott, Roland K. Perkins |
|---|---|
| Rok vydání: | 1964 |
| Předmět: |
Physiology
medicine.medical_treatment Urine Blood–brain barrier Peak concentration Dogs Cerebrospinal fluid Developmental Neuroscience medicine Animals Mannitol Radiometry Saline Cerebrospinal Fluid Brain Chemistry Carbon Isotopes Chromatography Chemistry Research Brain Penetration (firestop) Metabolism medicine.anatomical_structure Neurology Blood-Brain Barrier lipids (amino acids peptides and proteins) medicine.drug |
| Zdroj: | Experimental Neurology. 10:264-270 |
| ISSN: | 0014-4886 |
| DOI: | 10.1016/0014-4886(64)90067-6 |
| Popis: | Fifty microcuries of radioactive labelled mannitol (d-mannitol-1-C14) was administered to each of eight dogs. In six it was mixed with unlabelled mannitol (3 gm/kg); in two, it was mixed with an equivalent volume of normal saline. Determination of concentration of radioactivity in serum, urine, CSF and brain was done at intervals up to 6 hours. Seventy-five per cent of the C14 introduced with inert mannitol was excreted in 6 hours. The peak concentration of radio-activity in the CSF was reached at about 2 hours from the start of the infusion. However, the serum radioactivity concentration at this time was considerably higher than CSF. The maximum ratio of CSF/serum concentrations of C14 was 0.37, at 6 hours, and the brain/serum ratio of C14 concentrations at this time was 0.62. If a small percentage of mannitol undergoes metabolism in the body, the actual concentration of mannitol may be even lower than assumed from counting the C14 tracer. At no time during the study of the six animals given tracer with unlabelled mannitol did the CSF or brain concentration of C14 approach that in the serum. |
| Databáze: | OpenAIRE |
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