Ribosome biogenesis is dynamically regulated during osteoblast differentiation
| Autor: | Xiaojing Mao, Amy E. Merrill, Creighton T. Tuzon, Cynthia L. Neben, Fides D. Lay |
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| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Transcription Genetic 5.8S ribosomal RNA Ribosome biogenesis Biology Article 03 medical and health sciences Mice 0302 clinical medicine Transcription (biology) Genetics Protein biosynthesis RNA polymerase I Animals Gene Osteoblasts RNA Cell Differentiation General Medicine 3T3 Cells Ribosomal RNA Molecular biology Cell biology 030104 developmental biology RNA Ribosomal 030220 oncology & carcinogenesis Protein Biosynthesis Ribosomes |
| Popis: | Changes in ribosome biogenesis are tightly linked to cell growth, proliferation, and differentiation. The rate of ribosome biogenesis is established by RNA Pol I-mediated transcription of ribosomal RNA (rRNA). Thus, rRNA gene transcription is a key determinant of cell behavior. Here, we show that ribosome biogenesis is dynamically regulated during osteoblast differentiation. Upon osteoinduction, osteoprogenitor cells transiently silence a subset of rRNA genes through a reversible mechanism that is initiated through biphasic nucleolar depletion of UBF1 and then RNA Pol I. Nucleolar depletion of UBF1 is coincident with an increase in the number of silent but transcriptionally permissible rRNA genes. This increase in the number of silent rRNA genes reduces levels of ribosome biogenesis and subsequently, protein synthesis. Together these findings demonstrate that fluctuations in rRNA gene transcription are determined by nucleolar occupancy of UBF1 and closely coordinated with the early events necessary for acquisition of the osteoblast cell fate. |
| Databáze: | OpenAIRE |
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