Comparative cytotoxicity induced by bulk and nanoparticulated ZnO in the fish and human hepatoma cell lines PLHC-1 and Hep G2
| Autor: | Tobias Lammel, Sylvain Derick, Yolanda Pérez, A.I. Barrado, Christophe Furger, Estefanía Conde, María Luisa Fernández-Cruz, Marta Fernández, José María Navas, Mona Connolly |
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| Jazyk: | angličtina |
| Rok vydání: | 2013 |
| Předmět: |
Materials science
Light Cell Survival Biomedical Engineering Nanoparticle Metal Nanoparticles Nanotechnology 010501 environmental sciences Toxicology medicine.disease_cause 01 natural sciences Mass Spectrometry 03 medical and health sciences In vitro Cell Line Tumor medicine Cytotoxic T cell ICP-MS Animals Humans Scattering Radiation Particle Size Cytotoxicity 030304 developmental biology 0105 earth and related environmental sciences 0303 health sciences Poecilia L-Lactate Dehydrogenase In vitro toxicology Hep G2 Cells Hep G2 Oxidative Stress Neutral Red Toxicity Biophysics LUCS Nanoparticles Zinc Oxide Reactive Oxygen Species Oxidative stress |
| Zdroj: | Repositorio de Resultados de Investigación del INIA Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria INIA INIA: Repositorio de Resultados de Investigación del INIA |
| Popis: | The increasing presence of ZnO nanoparticles (NPs) in consumer products may be having a dramatic impact in aquatic environments. The evaluation of ZnO NP toxicity represents a great challenge. This study aimed at evaluating the cytotoxic effect of micro-and nanosized ZnO in a fish and a mammalian hepatoma cell line. A detailed characterisation of the particles in exposure media showed that ZnO NPs formed large aggregates. ZnO cytotoxicity was evaluated with a battery of in vitro assays including LUCS, a new approach based on DNA alteration measurements. In fish cells, ZnO NP aggregates contributed substantially to the cytotoxic effects whereas toxicity in the human cells appeared to be mainly produced by the dissolved fraction. ROS production did not contribute to the observed cytotoxicity. This work also showed that measuring concentrations of NPs is essential to understand the mechanisms underlying their toxicity. © 2013 Informa UK, Ltd. |
| Databáze: | OpenAIRE |
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