Microparticle-mediated thrombin generation assay: increased activity in patients with recurrent thrombosis
| Autor: | J. J. Jimenez, Carlos Bidot, Yeon-Soong Ahn, Loreta Bidot, W. Jy, Lawrence L. Horstman, V. Fontana |
|---|---|
| Rok vydání: | 2008 |
| Předmět: |
Adult
Blood Platelets Male medicine.medical_specialty Erythrocytes Cell Flow cytometry Thromboplastin Andrology Tissue factor Thrombin medicine Leukocytes Humans Platelet Microparticle Aged Aged 80 and over medicine.diagnostic_test Chemistry Anticoagulants Endothelial Cells Thrombosis Hematology Middle Aged medicine.disease Surgery medicine.anatomical_structure Case-Control Studies Female medicine.drug |
| Zdroj: | Journal of thrombosis and haemostasis : JTH. 6(6) |
| ISSN: | 1538-7836 |
| Popis: | Summary. Background: Circulating cell-derived microparticles (MP) are important players in thrombogenesis, attributed in part to tissue factor (TF) carried on them. We developed MP-mediated thrombin generation assay (TGA) and measured a series of patients with thrombosis (TBS) and normal controls (NC). Methods: MP were isolated from plasma of 66 patients with TBS and 34 NC. The MP were resuspended in normal pooled particle-free plasma (PFP) containing corn trypsin inhibitor (to inhibit contact pathway). MP mediated TGA yields three parameters: lag time, peak and rate. This method is not influenced by anticoagulant therapy. Of the TBS patients, 41 had only a single thrombosis (S-TBS) and 25 had recurrences (R-TBS) within a 5-year period. In parallel, MP were quantitated by flow cytometry, and cell origin was determined: endothelial cells (EMP), leukocytes (LMP), red cells (RMP) and platelets (PMP). Results: MP from all TBS patients exhibited higher thrombin generation than NC by all three TGA parameters. R-TBS had significantly greater TGA values than S-TBS, reflected in higher peak and rate, and shorter lag time. MP numbers were also higher in TBS vs. NC, for all MP subtypes, and were significantly higher in R-TBS than S-TBS (except LMP). All MP levels correlated with thrombin generation (P |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Plný text