Effects of sulphur and Thiobacillus thioparus 1904 on nitrogen cycle genes during chicken manure aerobic composting
| Autor: | Peizhi Xu, Dan Wang, Wu Hangtao, Sun Lili, Wenjie Gu, Chaohong Shi, Lu Yusheng, Li Xia, Kaizhi Xie |
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| Rok vydání: | 2018 |
| Předmět: |
inorganic chemicals
0301 basic medicine Denitrification Nitrogen 030106 microbiology Nitrous-oxide reductase 010501 environmental sciences 01 natural sciences 03 medical and health sciences chemistry.chemical_compound Animals Food science Nitrite Waste Management and Disposal Nitrogen cycle 0105 earth and related environmental sciences Composting Ammonia monooxygenase Nitrogen Cycle Thiobacillus Manure Nitrite oxidoreductase chemistry Chicken manure Nitrification Chickens Sulfur |
| Zdroj: | Waste management (New York, N.Y.). 80 |
| ISSN: | 1879-2456 |
| Popis: | Severe nitrogen (N) loss is a barrier for composting treatment. Since N transformation during composting is closely related to nitrogen loss, the impacts of adding sulphur and Thiobacillus thioparus 1904 to N transformation during composting were investigated in this work. Physicochemical properties and the expression of genes encoding N-related proteins were analysed to evaluate microbiological processes associated with N dynamics. The results indicated that (1) sulphur addition reduced the pH and cumulative NH3 emission, and decreased N losses by 44.23%, while no significant differences were observed in the expression of N cycle-associated genes compared with the control treatment; (2) the application of T. thioparus 1904 increased NO3−-N content, reduced N loss by 28.20%, and significantly enhanced the expression of ammonia monooxygenase A (archaeal amoA; AOA) and nitrite oxidoreductase A (nxrA) during the mature phase; (3) the combined application of sulphur and T. thioparus 1904 significantly affected the expression of functional genes related to nitrification and denitrification, which contributed to a reduction in accumulated NH3 emission, an increase in NO4+-N content, and a decrease in N losses by 70.94%. Expression of ammonia monooxygenase A (bacterial amoA; AOB), nxrA and nitrous oxide reductase Z (nosZ) genes in the combined treatment was positively correlated with NO3−-N, whereas expression of AOA and accumulation of NH3 were negatively correlated with NO3−-N. These results indicate that the combined application of sulphur and T. thioparus 1904 had a significant regulatory effect on N cycle genes and effectively reduced the N loss during composting. |
| Databáze: | OpenAIRE |
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