Molecular detection of virulence factors among food and clinical Enterococcus faecalis strains in South Brazil
| Autor: | Pedro Alves d'Azevedo, Ana Paula Guedes Frazzon, Aline Weber Medeiros, Daniele Vargas de Oliveira, Rebeca Inhoque Pereira, Jeverson Frazzon, S. T. Van Der Sand, Paula Dalcin Martins |
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| Rok vydání: | 2014 |
| Předmět: |
Virulence Factors
lcsh:QR1-502 Virulence Biology Hemolysis Microbiology lcsh:Microbiology Virulence factor Enterococcus faecalis Bacterial Proteins Humans Food microbiology Gelatinase Gene Gram-Positive Bacterial Infections Biofilme Biofilm biology.organism_classification QR1-502 clinical enterococci Virulência food enterococci Gelatinases Biofilms Food Microbiology biofilm formation Cytolysin virulence determinants Brazil Research Paper |
| Zdroj: | Brazilian Journal of Microbiology v.45 n.1 2014 Brazilian Journal of Microbiology Sociedade Brasileira de Microbiologia (SBM) instacron:SBM Brazilian Journal of Microbiology, Volume: 45, Issue: 1, Pages: 327-332, Published: 18 APR 2014 Brazilian Journal of Microbiology, Vol 45, Iss 1, Pp 327-332 (2014) Repositório Institucional da UFRGS Universidade Federal do Rio Grande do Sul (UFRGS) instacron:UFRGS |
| ISSN: | 1517-8382 |
| DOI: | 10.1590/s1517-83822014005000031 |
| Popis: | The present report aimed to perform a molecular epidemiological survey by investigating the presence of virulence factors in E. faecalis isolated from different human clinical (n = 57) and food samples (n = 55) in Porto Alegre, Brazil, collected from 2006 to 2009. In addition, the ability to form biofilm in vitro on polystyrene and the β-haemolytic and gelatinase activities were determined. Clinical strains presented a higher prevalence of aggregation substance (agg), enterococcal surface protein (esp) and cytolysin (cylA) genes when compared with food isolates. The esp gene was found only in clinical strains. On the other hand, the gelatinase (gelE) and adherence factor (ace) genes had similar prevalence among the strains, showing the widespread occurrence of these virulence factors among food and clinical E. faecalis strains in South Brazil. More than three virulence factor genes were detected in 77.2% and 18.2% of clinical and food strains, respectively. Gelatinase and β-haemolysin activities were not associated with the presence of gelE and cylA genes. The ability to produce biofilm was detected in 100% of clinical and 94.6% of food isolates, and clinical strains were more able to form biofilm than the food isolates (Student’s t-test, p < 0.01). Results from the statistical analysis showed significant associations between strong biofilm formation and ace (p = 0.015) and gelE (p = 0.007) genes in clinical strains. In conclusion, our data indicate that E. faecalis strains isolated from clinical and food samples possess distinctive patterns of virulence factors, with a larger number of genes that encode virulence factors detected in clinical strains. |
| Databáze: | OpenAIRE |
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