Mosquito-Bacteria Symbiosis: The Case of Anopheles gambiae and Asaia
| Autor: | Mauro Mandrioli, Ingrid Faye, Marco Genchi, N. Sagnon, Daniele Daffonchio, Cheryl Whitehorn, Claudia Damiani, Guelbeogo W. Moussa, Fulvio Esposito, Paolo Rossi, Guido Favia, Elena Crotti, Patrizia Scuppa, Angray S. Kang, Luciano Sacchi, Ulisse Ulissi, Aida Capone, Bessem Chouaia, Aurora Rizzi, Irene Ricci, Claudio Bandi, Sara Epis |
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| Rok vydání: | 2010 |
| Předmět: |
DNA
Bacterial Evolution Anopheles gambiae malaria Soil Science Asaia Paratransgenesis Biology Settore BIO/19 - Microbiologia Generale Polymerase Chain Reaction paratransgenesis law.invention Microbiology 03 medical and health sciences Transformation Genetic Behavior and Systematics law RNA Ribosomal 16S Anopheles parasitic diseases medicine Animals Parasite hosting Symbiosis Ecology Evolution Behavior and Systematics Ecology Anopheles stephensi Phylogeny Polymerase chain reaction 030304 developmental biology 0303 health sciences Organisms Genetically Modified 030306 microbiology Ovary Midgut Sequence Analysis DNA Ribosomal RNA biology.organism_classification medicine.disease 3. Good health symbiotic control Acetobacteraceae Female Settore AGR/11 - Entomologia Generale e Applicata Malaria |
| Zdroj: | Microbial Ecology. 60:644-654 |
| ISSN: | 1432-184X 0095-3628 |
| DOI: | 10.1007/s00248-010-9704-8 |
| Popis: | The symbiotic relationship between Asaia, an α-proteobacterium belonging to the family Acetobacteriaceae, and mosquitoes has been studied mainly in the Asian malaria vector Anopheles stephensi. Thus, we have investigated the nature of the association between Asaia and the major Afro-tropical malaria vector Anopheles gambiae. We have isolated Asaia from different wild and laboratory reared colonies of A. gambiae, and it was detected by PCR in all the developmental stages of the mosquito and in all the specimens analyzed. Additionally, we have shown that it localizes in the midgut, salivary glands and reproductive organs. Using recombinant strains of Asaia expressing fluorescent proteins, we have demonstrated the ability of the bacterium to colonize A. gambiae mosquitoes with a pattern similar to that described for A. stephensi. Finally, fluorescent in situ hybridization on the reproductive tract of females of A. gambiae showed a concentration of Asaia at the very periphery of the eggs, suggesting that transmission of Asaia from mother to offspring is likely mediated by a mechanism of egg-smearing. We suggest that Asaia has potential for use in the paratransgenic control of malaria transmitted by A. gambiae. |
| Databáze: | OpenAIRE |
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