Membrane labeling and immobilization via copper-free click chemistry
| Autor: | Matthew D. Smith, Michael D. Best, Heidi E. Bostic, Andrei Poloukhtine, Vladimir V. Popik |
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| Rok vydání: | 2011 |
| Předmět: |
Azides
Catalysis chemistry.chemical_compound Cyclooctanes Materials Chemistry Organic chemistry Molecule Derivatization Liposome Molecular Structure Staining and Labeling Metals and Alloys General Chemistry Combinatorial chemistry Lipids Surfaces Coatings and Films Electronic Optical and Magnetic Materials Membrane chemistry Reagent Liposomes Ceramics and Composites Click chemistry Click Chemistry Streptavidin Bioorthogonal chemistry Conjugate |
| Zdroj: | Chemical communications (Cambridge, England). 48(10) |
| ISSN: | 1364-548X |
| Popis: | Copper-free click chemistry was employed to derivatize membrane bilayers. This approach uses an azido-lipid conjugate presented on liposomes, which can be labeled in bioorthogonal fashion via cyclooctyne-tagged reagents. An immobilization-based approach using streptavidin-coated microplates was exploited to evaluate membrane derivatization. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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