Gram-positive and gram-negative bacteria induce different patterns of cytokine production in human mononuclear cells irrespective of taxonomic relatedness
| Autor: | Christina Hessle, Lars Hynsjö, Ingar Olsen, Agnes E. Wold, Floyd E. Dewhirst, Wilhelm Tham, Susann Skovbjerg, Anna Martner |
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| Rok vydání: | 2009 |
| Předmět: |
Gram-negative bacteria
Ultraviolet Rays Gram-positive bacteria medicine.medical_treatment Immunology Enzyme-Linked Immunosorbent Assay Gram-Positive Bacteria Lymphocyte Activation Peripheral blood mononuclear cell Microbiology Immune system Antigen Phagocytosis Species Specificity Virology Gram-Negative Bacteria medicine Humans Cells Cultured Antigens Bacterial Innate immune system biology Cell Biology biology.organism_classification Immunity Innate Cytokine Leukocytes Mononuclear Cytokines Tumor necrosis factor alpha Inflammation Mediators |
| Zdroj: | Journal of interferoncytokine research : the official journal of the International Society for Interferon and Cytokine Research. 30(1) |
| ISSN: | 1557-7465 |
| Popis: | Upon bacterial stimulation, tissue macrophages produce a variety of cytokines that orchestrate the immune response that clears the infection. We have shown that Gram-positives induce higher levels of interleukin-12 (IL-12), interferon-gamma (IFN-gamma), and tumor necrosis factor (TNF) from human peripheral blood mononuclear cells (PBMCs) than do Gram-negatives, which instead induce more of IL-6, IL-8, and IL-10. Here, we study whether these patterns follows or crosses taxonomic borders. PBMCs from blood donors were incubated with UV-inactivated bacteria representing 37 species from five phyla. IL-12, TNF, IL-1beta, IL-6, IL-8, and IL-10 were measured in the supernatants after 24 h and IFN-gamma after 5 days. Irrespective of phylogenetic position, Gram-positive bacteria induced much more IL-12 (nine times more on average) and IFN-gamma (seven times), more TNF (three times), and slightly more IL-1beta (1.5 times) than did Gram-negatives, which instead induced more IL-6 (1.5 times), IL-8 (1.9 times), and IL-10 (3.3 times) than did Gram-positives. A notable exception was the Gram-positive Listeria monocytogenes, which induced very little IL-12, IFN-gamma, and TNF. The results confirm the fundamental difference in innate immune responses to Gram-positive and Gram-negative bacteria, which crosses taxonomic borders and probably reflects differences in cell wall structure. |
| Databáze: | OpenAIRE |
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