HPTLC chromatography of androstene derivates. Application of normal phase thin-layer chromatographic retention data in QSAR studies
| Autor: | Nada Perišić-Janjić, Katarina Penov-Gaši, Tatjana Djaković-Sekulić, Srdjan Z. Stojanović |
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| Rok vydání: | 2004 |
| Předmět: |
Models
Molecular Quantitative structure–activity relationship Acetonitriles Hot Temperature Clinical Biochemistry Ethyl acetate Substituent Quantitative Structure-Activity Relationship Acetates Biochemistry High-performance liquid chromatography chemistry.chemical_compound Endocrinology Acetonitrile Benzene Molecular Biology Chromatography High Pressure Liquid Pharmacology Chromatography Silica gel Organic Chemistry Molecular Weight chemistry Models Chemical Lipophilicity Androstenes Chromatography Thin Layer |
| Zdroj: | Steroids. 70(3) |
| ISSN: | 0039-128X |
| Popis: | The chromatographic behavior of seven 16-oximino derivatives of 3beta-hydropxy-5-androstene have been investigated using the normal-phase (NP) HPTLC chromatographic mode of the type silica-non-polar diluent (benzene)-polar modifier (acetonitrile, ethyl acetate, or dioxane). The linear relationship between the retention constants (R(M)) and the logarithm of the organic modifier content in the mobile phase allowed for the calculation of R(M)0 values. The influence of substituent in the molecule on extrapolated retention data is discussed. To better understand the retention mechanism in the separation of androstene compounds, the functional group contributions (tauX) were compared with Hansch substituent constants (pi). An attempt to quantitate the lipophilicity of the investigated compounds using normal phase thin-layer chromatographic R(M)0 value was made. Also, the relative lipophilicity values determined previously by RPC as well as activity were compared with NPC data. |
| Databáze: | OpenAIRE |
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