A serological survey of Bacillus anthracis reveals widespread exposure to the pathogen in free-range and captive lions in Zimbabwe
| Autor: | Michel De Garine-Wichatitsky, Gift Matope, Andrew J. Loveridge, Davies M. Pfukenyi, Okechukwu C. Ndumnego, Norman L. Mukarati, Alexandre Caron, Sunday O. Ochai, Henriette van Heerden, Solomon Jauro, Tapiwanashe Hanyire |
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| Přispěvatelé: | University of Zimbabwe (UZ), University of Pretoria [South Africa], Moredun Research Institute [Penicuik, UK] (MRI), University of Oxford [Oxford], Animal, Santé, Territoires, Risques et Ecosystèmes (UMR ASTRE), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Eduardo Mondlane University, Kasetsart University - KU (THAILAND), Kasetsart University (KU), Ministere Francais des Affaires Etrangeres, Grant/Award Number: MinistereFSP-RenCaRe project (FSP no2011/36)., University of Zimbabwe Research Board, Grant/Award Number: RB40/2015, University of Zimbabwe |
| Rok vydání: | 2020 |
| Předmět: |
Veterinary medicine
[SDV]Life Sciences [q-bio] L73 - Maladies des animaux Neutralization Serology 0403 veterinary science Réserve naturelle Seroepidemiologic Studies Epidemiology anthrax protective antibody-antigen Prevalence toxin neutralization assay (TNA) anthrax (Bacillus anthracis) 0303 health sciences biology medicine.diagnostic_test 04 agricultural and veterinary sciences General Medicine Antibodies Bacterial Bacillus anthracis Test ELISA Lion Livestock Immunodiagnostic Antibody Lions Zimbabwe medicine.medical_specialty 040301 veterinary sciences Animals Wild Enzyme-Linked Immunosorbent Assay Anthrax Technique immunoenzymatique 03 medical and health sciences biology.animal medicine Animals 030304 developmental biology captive game parks [SDV.BA.MVSA]Life Sciences [q-bio]/Animal biology/Veterinary medicine and animal Health General Veterinary General Immunology and Microbiology business.industry fungi Outbreak Animal sauvage biology.organism_classification Virology Antibodies Neutralizing ELISA immunoassay African lions (Panthera leo) Enquête pathologique Immunoassay biology.protein [SDV.SPEE]Life Sciences [q-bio]/Santé publique et épidémiologie Animals Zoo animal sauvage captif protected areas Panthera business |
| Zdroj: | Transboundary and emerging diseases Transboundary and emerging diseases, Wiley-Blackwell, 2021, 68 (3), pp.1676-1684. ⟨10.1111/tbed.13842⟩ Transboundary and Emerging Diseases |
| ISSN: | 1865-1682 1865-1674 |
| DOI: | 10.1111/tbed.13842⟩ |
| Popis: | International audience; Numerous unknown factors influence anthrax epidemiology in multi-host systems, especially at wildlife/livestock/human interfaces. Serology tests for anti-anthrax antibodies in carnivores are useful tools in identifying the presence or absence ofBacillus anthracisin a range. These were employed to ascertain whether the disease pattern followed the recognized high- and low-risk anthrax zonation in Zimbabwe and also to establish whether anthrax was absent from Hwange National Park in which there have been no reported outbreaks. African lions (Panthera leo)(n = 114) drawn from free-range protected areas and captive game parks located in recognized high- and low-risk zones across Zimbabwe were tested for antibodies to anthrax PA antigen using the ELISA immunoassay. A random selection of 27 lion sera samples comprising 17 seropositive and 10 seronegative sera was further tested in the species-independent toxin neutralization assay (TNA) in order to validate the former as a surveillance tool for anthrax in African lions. Using the ELISA-PA immunoassay, 21.9% (25/114) of the lions tested positive for antibodies to anthrax. Seropositivity was recorded in all study areas, and there was no significant difference (p = .852) in seropositivity between lions in high- and low-risk anthrax zones. Also, there was no significant difference (McNemar's chi-square test = 0.9,p = .343) in the proportion of lions testing positive to anti-PA anthrax antibodies on ELISA-PA immunoassay compared with the TNA, with fair agreement between the two tests [kappa (K) statistic = 0.30; 0.08 < K |
| Databáze: | OpenAIRE |
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