A Fine-Structure Map of Spontaneous Mitotic Crossovers in the Yeast Saccharomyces cerevisiae
| Autor: | Phoebe S. Lee, Malgorzata Gawel, Margaret Dominska, Patricia W. Greenwell, Thomas D. Petes, Monica L. Hamilton |
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| Rok vydání: | 2009 |
| Předmět: |
Cancer Research
Mitotic crossover lcsh:QH426-470 DNA Repair Mitosis Saccharomyces cerevisiae Biology Chromosomal crossover Loss of heterozygosity 03 medical and health sciences 0302 clinical medicine Genetics Gene conversion Genetics and Genomics/Cancer Genetics Molecular Biology Genetics (clinical) Ecology Evolution Behavior and Systematics 030304 developmental biology Recombination Genetic 0303 health sciences G1 Phase Chromosome Mapping Chromosome Genetics and Genomics/Microbial Evolution and Genomics Genetics and Genomics/Chromosome Biology lcsh:Genetics Genetics and Genomics/Genome Projects Perspective Chromatid Chromosomes Fungal Homologous recombination 030217 neurology & neurosurgery |
| Zdroj: | PLoS Genetics PLoS Genetics, Vol 5, Iss 3, p e1000410 (2009) |
| ISSN: | 1553-7404 |
| DOI: | 10.1371/journal.pgen.1000410 |
| Popis: | Homologous recombination is an important mechanism for the repair of DNA damage in mitotically dividing cells. Mitotic crossovers between homologues with heterozygous alleles can produce two homozygous daughter cells (loss of heterozygosity), whereas crossovers between repeated genes on non-homologous chromosomes can result in translocations. Using a genetic system that allows selection of daughter cells that contain the reciprocal products of mitotic crossing over, we mapped crossovers and gene conversion events at a resolution of about 4 kb in a 120-kb region of chromosome V of Saccharomyces cerevisiae. The gene conversion tracts associated with mitotic crossovers are much longer (averaging about 12 kb) than the conversion tracts associated with meiotic recombination and are non-randomly distributed along the chromosome. In addition, about 40% of the conversion events have patterns of marker segregation that are most simply explained as reflecting the repair of a chromosome that was broken in G1 of the cell cycle. |
| Databáze: | OpenAIRE |
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