Clinical comparison of new monoclonal antibody-based nephelometric assays for free light chain kappa and lambda to polyclonal antibody-based assays and immunofixation electrophoresis
| Autor: | Rein M.J. Hoedemakers, Peter Stam, Roel Melsert, Johannes F.M. Pruijt, Elisa Teunissen, Henk Martens, Suzanne Hol, Henk te Velthuis |
|---|---|
| Přispěvatelé: | Landsteiner Laboratory |
| Jazyk: | angličtina |
| Rok vydání: | 2012 |
| Předmět: |
Electrophoresis
medicine.drug_class Clinical Biochemistry Paraproteinemias Reference range Monoclonal antibody Lambda Sensitivity and Specificity Immunoglobulin kappa-Chains Immunoglobulin lambda-Chains Nephelometry and Turbidimetry hemic and lymphatic diseases medicine Humans Serologic Tests Chromatography biology Chemistry Biochemistry (medical) Antibodies Monoclonal General Medicine Immunofixation electrophoresis Molecular biology Hospitals Polyclonal antibodies biology.protein Antibody Nephelometry Kappa |
| Zdroj: | Clinical chemistry and laboratory medicine, 50(3), 489-495. Walter de Gruyter GmbH |
| ISSN: | 1434-6621 |
| Popis: | Background: New monoclonal antibody-based assays for serum-free light chains (FLC) have become available. Methods: In a clinical study with 541 patients, the new N Latex FLC assays were compared with the Freelite (TM) FLC assays and immunofixation electrophoresis (IF). Results: Comparison of the different FLC kappa (kappa) assays showed a slope of 0.99 with a deviation of 5.0%, r(s)=0.92, for FLC lambda (lambda) a slope of 1.22, deviation 13.8%, r(s)=0.90 and for the kappa/lambda ratio a slope of 0.72, deviation -4.6%, r(s)=0.72. The concordance for the FLC kappa assays was 91%, for FLC lambda 85% and kappa/lambda ratio 95%. The clinical sensitivity and specificity of the kappa/lambda ratios in the study were comparable: 60% and 99% for the N Latex FLC assay and 61% and 97% for the Freelite (TM) assay. In IF-FLC positive samples, the N Latex FLC kappa/lambda ratio scored 20/23 (87%) samples outside the reference range and Freelite (TM) 21/23 (91%). For IF-FLC negative samples, N Latex FLC assay kappa/lambda ratio scored 338/350 (97%) within the reference range and Freelite (TM) scored 332/350 (95%). Conclusions: The concordance scores and the clinical sensitivity and specificity of the new N Latex FLC assays and Freelite (TM) assays appeared comparable, but there are some differences in measurement of concentrations between the methods |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|