Use of a Whole Genome Approach To Identify Vaccine Molecules Affording Protection against Streptococcus pneumoniae Infection
Autor: | Craig A. Rosen, Yambasu A. Brewah, Wizemann Theresa M, Syd Johnson, Philip Barren, Solomon Langermann, Raju Lathigra, Jon Heinrichs, William Walsh, H. Robert Masure, Charles A. Kunsch, Anthony Gayle, John E. Adamou, Gil H. Choi, Scott Koenig, Steven C. Barash, Elaine Tuomanen, Mark S. Hanson, Alice L. Erwin |
---|---|
Rok vydání: | 2001 |
Předmět: |
Molecular Sequence Data
Immunology Virulence Biology medicine.disease_cause Microbiology Pneumococcal Infections Pneumococcal Vaccines Mice Immune system Sepsis Streptococcus pneumoniae medicine Animals Humans Technology Pharmaceutical Amino Acid Sequence Serotyping Pathogen Conserved Sequence Antigens Bacterial Mice Inbred C3H Immunogenicity Reverse vaccinology Convalescence Genomics Virology Bacterial vaccine Infectious Diseases Microbial Immunity and Vaccines Bacterial Vaccines Humoral immunity Female Parasitology |
Zdroj: | Infection and Immunity. 69:1593-1598 |
ISSN: | 1098-5522 0019-9567 |
Popis: | Microbial targets for protective humoral immunity are typically surface-localized proteins and contain common sequence motifs related to their secretion or surface binding. Exploiting the whole genome sequence of the human bacterial pathogen Streptococcus pneumoniae , we identified 130 open reading frames encoding proteins with secretion motifs or similarity to predicted virulence factors. Mice were immunized with 108 of these proteins, and 6 conferred protection against disseminated S. pneumoniae infection. Flow cytometry confirmed the surface localization of several of these targets. Each of the six protective antigens showed broad strain distribution and immunogenicity during human infection. Our results validate the use of a genomic approach for the identification of novel microbial targets that elicit a protective immune response. These new antigens may play a role in the development of improved vaccines against S. pneumoniae . |
Databáze: | OpenAIRE |
Externí odkaz: |