Effects of the pathological E200K mutation on human prion protein: A computational screening and molecular dynamics approach

Autor: Fatemeh Rahimi Gharemirshamloo, Ranabir Majumder, Udhaya Kumar S., George Priya Doss C., Kourosh Bamdad, Fateme Frootan, Cemal Un
Rok vydání: 2022
Předmět:
Zdroj: Journal of Cellular Biochemistry. 124:254-265
ISSN: 1097-4644
0730-2312
Popis: The human prion protein gene (PRNP) is mapped to the short arm of chromosome 20 (20pter-12). Prion disease is associated with mutations in the prion protein-encoding gene sequence. Earlier studies found that the mutation G127V in the PRNP increases protein stability. In contrast, the mutation E200K, which has the highest mutation rate in the prion protein, causes Creutzfeldt-Jakob disease (CJD) in humans and induces protein aggregation. We aimed to identify the structural mechanisms of E200k and G127V mutations causing CJD. We used a variety of bioinformatic algorithms, including SIFT, PolyPhen, I-Mutant, PhD-SNP, and SNP& GO, to predict the association of the E200K mutation with prion disease. MD simulation is performed, and graphs for root mean square deviation, root mean square fluctuation, radius of gyration, DSSP, principal component analysis, porcupine, and free energy landscape are generated to confirm and prove the stability of the wild-type and mutant protein structures. The protein is analyzed for aggregation, and the results indicate more fluctuations in the protein structure during the simulation owing to the E200K mutation; however, the G127V mutation makes the protein structure stable against aggregation during the simulation.
Databáze: OpenAIRE
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