Return of Widespread Chloroquine-Sensitive Plasmodium falciparum to Malawi
| Autor: | Jacek Skarbinski, Cassidy W. Claassen, Shannon Takala-Harrison, Anne E. P. Frosch, Miriam K. Laufer, Don P. Mathanga, Fraction K. Dzinjalamala, Christopher V. Plowe |
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| Rok vydání: | 2014 |
| Předmět: |
Male
Malawi Genotype Genotyping Techniques Plasmodium falciparum Protozoan Proteins macromolecular substances Parasitemia Antimalarials Major Articles and Brief Reports Parasitic Sensitivity Tests Chloroquine parasitic diseases medicine Humans Immunology and Allergy Malaria Falciparum Geographic area biology Infant Membrane Transport Proteins Sequence Analysis DNA biology.organism_classification medicine.disease Virology Cross-Sectional Studies Infectious Diseases Child Preschool Immunology Pyrosequencing Malaria prevention Malaria medicine.drug |
| Zdroj: | The Journal of Infectious Diseases. 210:1110-1114 |
| ISSN: | 1537-6613 0022-1899 |
| DOI: | 10.1093/infdis/jiu216 |
| Popis: | Background. The return of chloroquine-sensitive Plasmodium falciparum to the limited area of Blantyre, Malawi, has been well demonstrated in several studies. Methods. To characterize chloroquine susceptibility over a wide geographic area, infants and children aged 6–59 months were selected using 2-stage cluster sampling in 8 Malawian districts. Pyrosequencing of the pfcrt gene codon 76 region was performed for children with asexual parasitemia. Results. Of 7145 children, 1150 had microscopic asexual parasitemia, and sequencing was performed in 685, of whom 1 had a chloroquine-resistant genotype. Conclusions. Systematic countrywide sampling demonstrates that the chloroquine pfcrt genotype has reached near-fixation, raising the possibility of reintroducing chloroquine for malaria prevention and treatment. |
| Databáze: | OpenAIRE |
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