Enriching for human acute myeloid leukemia stem cells using reactive oxygen species-based cell sorting
| Autor: | Craig T. Jordan, Courtney L. Jones, Cristiana O’Brien, Brett M. Stevens |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Mice
SCID Stem cells Biology General Biochemistry Genetics and Molecular Biology Mice Antigen Mice Inbred NOD Protocol medicine Animals Humans lcsh:Science (General) Cancer chemistry.chemical_classification Reactive oxygen species General Immunology and Microbiology General Neuroscience Myeloid leukemia Cell sorting Flow Cytometry medicine.disease Leukemia Myeloid Acute Leukemia Metabolism chemistry Neoplastic Stem Cells Cancer research Stem cell Reactive Oxygen Species Flow cytometry/mass cytometry lcsh:Q1-390 |
| Zdroj: | STAR Protocols, Vol 2, Iss 1, Pp 100248-(2021) STAR Protocols |
| ISSN: | 2666-1667 |
| Popis: | Summary Isolation of leukemia stem cells presents a challenge due to the heterogeneity of the immunophenotypic markers commonly used to identify blood stem cells. Several studies have reported that relative levels of reactive oxygen species (ROS) can be used to enrich for stem cell populations, suggesting a potential alternative to surface antigen-based methods. Here, we describe a protocol to enrich for stem cells from human acute myeloid leukemia specimens using relative levels of ROS. This protocol provides consistent enrichment of leukemia stem cells. For complete details on the use and execution of this protocol, please refer to Lagadinou et al. (2013) and Pei et al. (2018). Graphical abstract Highlights • Low levels of reactive oxygen species are a hallmark of stem cells • Relative levels of reactive oxygen species allow for enrichment of leukemia stem cells • This protocol is an alternative to surface antigen-based methods Isolation of leukemia stem cells presents a challenge due to the heterogeneity of the immunophenotypic markers commonly used to identify blood stem cells. Several studies have reported that relative levels of reactive oxygen species (ROS) can be used to enrich for stem cell populations, suggesting a potential alternative to surface antigen-based methods. Here, we describe a protocol to enrich for stem cells from human acute myeloid leukemia specimens using relative levels of ROS. This protocol provides consistent enrichment of leukemia stem cells. |
| Databáze: | OpenAIRE |
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