Maternal Sall4 Is Indispensable for Epigenetic Maturation of Mouse Oocytes
| Autor: | Yong Zhang, Yanhong Zhao, Xiaochen Kou, Chenfei Wang, Jiayu Chen, Kai Xu, Hong Wang, Linfeng Zhang, Zhenzhen Hou, Wenqiang Liu, Xia Chen, Yi-Wen Xu, Jingyi Li, Hui Yang, Fengchao Wang, Baodong Liu, Yuanlin He, Hua Huang, Yawei Gao, Kun Zhao, Shaorong Gao, Hailin Wang, Jing Li, Heng-Yu Fan |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Jumonji Domain-Containing Histone Demethylases Biology Biochemistry Oogenesis Epigenesis Genetic Mice 03 medical and health sciences Meiosis Histone methylation medicine Animals Epigenetics Molecular Biology Histone Demethylases Mice Knockout Genetics Germinal vesicle Cell Biology DNA Methylation Oocyte eye diseases Cell biology DNA-Binding Proteins 030104 developmental biology medicine.anatomical_structure DNA methylation Oocytes H3K4me3 Female Transcription Factors Developmental Biology |
| Zdroj: | Journal of Biological Chemistry. 292:1798-1807 |
| ISSN: | 0021-9258 |
| DOI: | 10.1074/jbc.m116.767061 |
| Popis: | Sall4 (Splat-like 4) plays important roles in maintaining pluripotency of embryonic stem cells and in various developmental processes. Here, we find that Sall4 is highly expressed in oocytes and early embryos. To investigate the roles of SALL4 in oogenesis, we generated Sall4 maternal specific knock-out mice by using CRISPR/Cas9 system, and we find that the maternal deletion of Sall4 causes developmental arrest of oocytes at germinal vesicle stage with non-surrounded nucleus, and the subsequent meiosis resumption is prohibited. We further discover that the loss of maternal Sall4 causes failure in establishment of DNA methylation in oocytes. Furthermore, we find that Sall4 modulates H3K4me3 and H3K27me3 modifications by regulating the expression of key histone demethylases coding genes Kdm5b, Kdm6a, and Kdm6b in oocytes. Moreover, we demonstrate that the aberrant H3K4me3 and H3K27me3 cause mis-expression of genes that are critical for oocytes maturation and meiosis resumption. Taken together, our study explores a pivotal role of Sall4 in regulating epigenetic maturation of mouse oocytes. |
| Databáze: | OpenAIRE |
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