Herpes Simplex Virus Type 1 Immediate-Early Protein ICP27 Is Required for Efficient Incorporation of ICP0 and ICP4 into Virions
| Autor: | Stephen A. Rice, Lenka Sedlackova |
|---|---|
| Rok vydání: | 2008 |
| Předmět: |
Cytoplasm
Ubiquitin-Protein Ligases viruses Immunology Herpesvirus 1 Human Biology Virus Replication medicine.disease_cause Microbiology Immediate early protein Immediate-Early Proteins Viral entry Virology Viral structural protein medicine Activator (genetics) Virus Assembly Virion Viral tegument biochemical phenomena metabolism and nutrition Genome Replication and Regulation of Viral Gene Expression Herpes simplex virus Viral replication Insect Science Mutant Proteins |
| Zdroj: | Journal of Virology. 82:268-277 |
| ISSN: | 1098-5514 0022-538X |
| DOI: | 10.1128/jvi.01588-07 |
| Popis: | Early in infection, herpes simplex virus type 1 (HSV-1) immediate-early (IE) proteins ICP0 and ICP4 localize to the nucleus, where they stimulate viral transcription. Later in infection, ICP0 and to a lesser extent ICP4 accumulate in the cytoplasm, but their biological role there is unknown. Previously, it was shown that the cytoplasmic localization of ICP0/4 requires the multifunctional IE protein ICP27, which is itself an activator of viral gene expression. Here, we identify a viral ICP27 mutant, d 3-4, which is unable to efficiently localize ICP0 and ICP4 to the cytoplasm but which otherwise resembles wild-type HSV-1 in its growth and viral gene expression phenotypes. These results genetically separate the function of ICP27 that affects ICP0/4 localization from its other functions, which affect viral growth and gene expression. As both ICP0 and ICP4 are known to be minor virion components, we used d 3-4 to test the hypothesis that the cytoplasmic localization of these proteins is required for their incorporation into viral particles. Consistent with this conjecture, d 3-4 virions were found to lack ICP0 in their tegument and to have greatly reduced levels of ICP4. Thus, the cytoplasmic localization of ICP0 and ICP4 appears to be a prerequisite for the assembly of these important transcriptional regulatory proteins into viral particles. Furthermore, our results show that ICP27 plays a previously unrecognized role in determining the composition of HSV-1 virions. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|