Intraoperative Photodynamic Diagnosis Using Talaporfin Sodium Simultaneously Applied for Photodynamic Therapy against Malignant Glioma: A Prospective Clinical Study
| Autor: | Kazuhide Shimizu, Masayuki Nitta, Takashi Komori, Takashi Maruyama, Takayuki Yasuda, Yu Fujii, Ken Masamune, Takakazu Kawamata, Taketoshi Maehara, Yoshihiro Muragaki |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
030103 biophysics medicine.medical_treatment Photodynamic diagnosis Photodynamic therapy NPe6 lcsh:RC346-429 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Glioma Fluorescence microscope medicine DAPI lcsh:Neurology. Diseases of the nervous system Original Research Chemistry business.industry glioblastoma talaporfin sodium malignant glioma medicine.disease Fluorescence Staining TALAPORFIN SODIUM Neurology photodynamic therapy Neurology (clinical) Nuclear medicine business 030217 neurology & neurosurgery Neuroscience photodynamic diagnosis |
| Zdroj: | Frontiers in Neurology Frontiers in Neurology, Vol 9 (2018) |
| ISSN: | 1664-2295 |
| Popis: | ObjectiveThe goal of this study was to demonstrate the feasibility of intraoperative photodynamic diagnosis (PDD) of malignant glioma using the fluorescence from talaporfin sodium (TS), which is used simultaneously for photodynamic therapy (PDT).MethodsPatients with suspected primary malignant glioma who were eligible for surgical removal of the tumor and PDT with TS were enrolled in this prospective study. Tissue samples were obtained from the contrast-enhanced (CE) region and from the surrounding non-contrast-enhanced (NCE) marginal tissue at the boundary between the tumor and normal tissue. The excised samples were set into a fluorescence measurement system, which consisted of a semiconductor laser with a 400-nm wavelength for excitation, and a compact spectrometer for detection, which were applied and received through a custom-made probe consisting of coaxial optical fibers. The fluorescence spectrum was obtained, and peak intensity was calculated. Tumor cellularity was histopathologically analyzed and semi-quantitatively classified into four (0–3) categories.Results86 samples from 17 surgical cases were available for fluorescence measurement and analysis. The fluorescence from TS had a single peak at 664 nm that was easily distinguished from the 400-nm excitation light. Samples from the CE regions showed higher fluorescence intensity than those from the NCE regions (P |
| Databáze: | OpenAIRE |
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