Enzymatic synthesis of vitamin B6 precursor
| Autor: | V Dusan Velickovic, D Zorica Knezevic-Jugovic, Z Nevena Prlainovic, Z Dusan Mijin, I Dejan Bezbradica |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2013 |
| Předmět: |
chemistry.chemical_classification
Addition reaction biology Chemistry 1-ethoxy-2 General Chemistry Candida rugosa lipase Enzymatic synthesis 010402 general chemistry 01 natural sciences pyridone 0104 chemical sciences Catalysis Candida rugosa 4-pentanedione lcsh:Chemistry Enzyme lcsh:QD1-999 biology.protein Organic chemistry ping-pong kinetics Vitamin b6 Lipase |
| Zdroj: | Journal of the Serbian Chemical Society, Vol 78, Iss 10, Pp 1491-1501 (2013) |
| ISSN: | 1820-7421 0352-5139 |
| Popis: | 3-Cyano-4-ethoxymethyl-6-methyl-2-pyridone is an important precursor in the synthesis of vitamin B6, obtained in the addition reaction between 2-cyanoacetamide and 1-ethoxy-2,4-pentanedione catalyzed by lipase from Candida rugosa (triacylglycerol ester hydrolases, EC 3.1.1.3). This work shows new experimental data and mathematical modeling of lipase catalyzed synthesis of 3-cyano-4-ethoxymethyl-6-methyl-2-pyridone, starting from 1-ethoxy-2,4-pentanedione and 2-cyanoacetamide. Kinetic measurements were done at 50 oC with enzyme concentration of 1.2 % w/v. Experimental results were fitted with two kinetic models: the ordered bi-ter and ping-pong bi-ter model, and the initial rates of the reaction were found to correlate best with a ping-pong bi-ter mechanism with inhibition by 2-cyanoacetamide. Obtained specificity constants indicated that lipase from C. rugosa had higher affinity towards 1-ethoxy-2,4-pentanedione and less bulky substrates. [Projekat Ministarstva nauke Republike Srbije, br. 172013, br. III 46010 and br. 172049] |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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