Evaluation of the BACTEC MGIT 960 system and the resazurin microtiter assay for susceptibility testing of Mycobacterium tuberculosis to second-line drugs
| Autor: | Rosangela Siqueira de Oliveira, Juliana Failde Gallo, Cláudia Patara Saraceni, Juliana Maira Watanabe Pinhata |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Microbiology (medical) Susceptibility testing Tuberculosis 030231 tropical medicine 030106 microbiology Antitubercular Agents Microbial Sensitivity Tests Proficiency test Drug resistance Reference laboratory Microbiology Mycobacterium tuberculosis 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Second line Oxazines Tuberculosis Multidrug-Resistant medicine Humans Molecular Biology Automation Laboratory biology business.industry Resazurin biology.organism_classification medicine.disease Xanthenes chemistry business |
| Zdroj: | Journal of Microbiological Methods. 139:168-171 |
| ISSN: | 0167-7012 |
| Popis: | Drug resistance in tuberculosis is a major threat to public health and control of the disease worldwide. Given the need of a rapid and accurate detection of Mycobacterium tuberculosis resistance to second-line drugs, this study evaluated the performance of the BACTEC MGIT 960 for second-line, drug susceptibility testing in comparison with the resazurin microtiter assay (REMA), in order to implement the automated methodology in the diagnostic routine of a reference laboratory. Drug susceptibility testing (DST) for second-line drugs of 151 MDR M. tuberculosis clinical isolates was performed by both BACTEC MGIT 960 and REMA, and a panel of 26 M. tuberculosis reference isolates from a proficiency test was tested by the BACTEC MGIT 960. DST for second-line drugs by the BACTEC MGIT 960 system was more rapid, highly reproducible and showed 100% of proficiency. After these results, this methodology was successfully implemented in our diagnostic routine for all MDR-TB patients. |
| Databáze: | OpenAIRE |
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