Iron is not everything: unexpected complex metabolic responses between iron-cycling microorganisms
| Autor: | Georg Pohnert, Kirsten Küsel, Carl-Eric Wegner, Remington X. Poulin, Jens D. Wurlitzer, Nico Ueberschaar, Stefan Kügler, Daniel Stettin, Thomas Wichard, Rebecca E. Cooper |
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| Rok vydání: | 2020 |
| Předmět: |
Shewanella
Hydrogenase Iron Microbiology Redox Article Microbial ecology Transcriptome 03 medical and health sciences Microaerophile Ferrous Compounds Shewanella oneidensis Ecology Evolution Behavior and Systematics 030304 developmental biology chemistry.chemical_classification 0303 health sciences biology 030306 microbiology Gallionellaceae Biofilm Biogeochemistry biology.organism_classification Amino acid Biochemistry chemistry Lipoprotein transport Oxidation-Reduction |
| Zdroj: | The ISME Journal |
| Popis: | Coexistence of microaerophilic Fe(II)-oxidizers and anaerobic Fe(III)-reducers in environments with fluctuating redox conditions is a prime example of mutualism, in which both partners benefit from the sustained Fe-pool. Consequently, the Fe-cycling machineries (i.e., metal-reducing or –oxidizing pathways) should be most affected during co-cultivation. However, contrasting growth requirements impeded systematic elucidation of their interactions. To disentangle underlying interaction mechanisms, we established a suboxic co-culture system of Sideroxydans sp. CL21 and Shewanella oneidensis. We showed that addition of the partner’s cell-free supernatant enhanced both growth and Fe(II)-oxidizing or Fe(III)-reducing activity of each partner. Metabolites of the exometabolome of Sideroxydans sp. CL21 are generally upregulated if stimulated with the partner´s spent medium, while S. oneidensis exhibits a mixed metabolic response in accordance with a balanced response to the partner. Surprisingly, RNA-seq analysis revealed genes involved in Fe-cycling were not differentially expressed during co-cultivation. Instead, the most differentially upregulated genes included those encoding for biopolymer production, lipoprotein transport, putrescine biosynthesis, and amino acid degradation suggesting a regulated inter-species biofilm formation. Furthermore, the upregulation of hydrogenases in Sideroxydans sp. CL21 points to competition for H2 as electron donor. Our findings reveal that a complex metabolic and transcriptomic response, but not accelerated formation of Fe-end products, drive interactions of Fe-cycling microorganisms. |
| Databáze: | OpenAIRE |
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