Combination of TLR2 and TLR3 agonists derepress infectious bursal disease virus vaccine-induced immunosuppression in the chicken

Autor:	Asok Kumar Mariappan, Saravanan Ramakrishnan, Javeed Ahmad Dar, Yogendra Singh, Deepthi Kappala, Khalid Bashir, Sohini Dey, Madhan Mohan Chellappa, Narayanan Krishnaswamy, Ashok Kumar, Tapas Kumar Goswami, Vivek Kumar Gupta
Rok vydání:	2019
Předmět:	0301 basic medicine animal structures lcsh:Medicine Nitric Oxide Synthase Type II Infectious bronchitis virus Nitric Oxide Peripheral blood mononuclear cell Infectious bursal disease virus Virus Article Infectious bursal disease 03 medical and health sciences Interferon-gamma Lipopeptides 0302 clinical medicine medicine Animals Adjuvants lcsh:Science Specific-pathogen-free Immunosuppression Therapy Multidisciplinary biology Macrophages lcsh:R Body Weight Toll-Like Receptors Viral Vaccines medicine.disease Avian infectious bronchitis biology.organism_classification Birnaviridae Infections Virology Toll-Like Receptor 2 Toll-Like Receptor 3 Interleukin 10 030104 developmental biology Poly I-C biology.protein Leukocytes Mononuclear lcsh:Q Antibody Chickens 030217 neurology & neurosurgery
Zdroj:	Scientific Reports Scientific Reports, Vol 9, Iss 1, Pp 1-16 (2019)
ISSN:	2045-2322
Popis:	Live intermediate plus infectious bursal disease virus (IBDV) vaccines (hot vaccines) are used for protection against the virulent IBDV strains in young chickens. We evaluated the potential of Toll-like receptor (TLR) agonists to alleviate hot vaccine-induced immunosuppression. The combination of Pam3CSK4 and poly I:C synergistically upregulated IFN-β, IFN-γ, IL-12, IL-4, and IL-13 transcripts and cross-inhibited IL-1β, IL-10, and iNOS transcripts in the chicken peripheral blood mononuclear cells (PBMCs) as analyzed by quantitative real-time PCR. Further, four-week old specific pathogen free White Leghorn chickens (n = 60) were randomly divided into six groups and either immunized with hot IBDV vaccine with or without Pam3CSK4 and/or poly I:C or not vaccinated to serve as controls. The results indicated that poly I:C alone and in combination with Pam3CSK4 alleviated vaccine-induced immunosuppression, as evidenced by greater weight gain, increased overall antibody responses to both sheep erythrocytes and live infectious bronchitis virus vaccine, upregulated IFN-γ transcripts and nitric oxide production by PBMCs (P 0.05), and lower bursal lesion score in the experimental birds. In conclusion, poly I:C alone and its combination with Pam3CSK4 reduced the destruction of B cells as well as bursal damage with restoration of function of T cells and macrophages when used with a hot IBDV vaccine.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::058a9bfc9618bc141c035dfc010e7ea7 https://pubmed.ncbi.nlm.nih.gov/31160675 Zobrazit plný text záznamu Plný text ve formátu PDF Plný text ve formátu HTML
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