Additional file 9: of IL-4 orchestrates STAT6-mediated DNA demethylation leading to dendritic cell differentiation

Autor: Vento-Tormo, Roser, Company, Carlos, RodríGuez-Ubreva, Javier, Rica, Lorenzo De La, JosÊ Urquiza, Biola Javierre, Radhakrishnan Sabarinathan, Luque, Ana, Esteller, Manel, Aran, Josep, ÁLvarez-Errico, Damiana, Ballestar, Esteban
Rok vydání: 2016
Předmět:
DOI: 10.6084/m9.figshare.c.3596765_d8
Popis: (A) Flow cytometry was used to detect surface marker changes in siRNA experiments during DC (-si STAT6 and -si JAK3) and MAC (-si STAT6) differentiation and maturation. A negative control pool from Dharmacon was used as a control in all experiments performed. (B) DNA methylation of MO transfected cells with an siRNA against STAT6 and their negative control, in the absence of IL-4 during MO differentiation. (C) Gene expression consequences measured by quantitative RT-PCR in DC, when STAT6 is inhibited by an siRNA. (PDF 26 kb)
Databáze: OpenAIRE